SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EIF2AK3 recombinant protein
EIF2AK3, Active
Applications
Western Blot
Synonyms
EIF2AK3; N/A; EIF2AK3, Active; PERK; PEK; WRS; HRI; DKFZp781H1925; EIF2AK3 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for EIF2AK3 recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for EIF2AK3 recombinant protein
Recombinant human EIF2AK3 (563-1115) was expressed in E Coli cells using an N-terminal GST tag. The gene accession number is NM_004836.
EIF2AK3 phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) leading to its inactivation and a rapid reduction of translational initiation and repression of global protein synthesis. EIF2AK3 is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins (1). EIF2AK3 plays a major role in the ability of cells to adapt to ER stress and is also involved in an integrated adaptive response to hypoxic stress in HeLa cells (2). EIF2AK3 functions in iron homeostasis and may play a role in hemolytic and inflammatory anemia.
EIF2AK3 phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) leading to its inactivation and a rapid reduction of translational initiation and repression of global protein synthesis. EIF2AK3 is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins (1). EIF2AK3 plays a major role in the ability of cells to adapt to ER stress and is also involved in an integrated adaptive response to hypoxic stress in HeLa cells (2). EIF2AK3 functions in iron homeostasis and may play a role in hemolytic and inflammatory anemia.
Product Categories/Family for EIF2AK3 recombinant protein
References
1. Harding, H. P. et.al: EIF2AK3 is essential for translational regulation and cell survival during the unfolded protein response. Molec. Cell 5: 897-904, 2000.
2. Blais, J. D. et.al: Activating transcription factor 4 is translationally regulated by hypoxic stress. Molec. Cell. Biol. 24: 7469-7482, 2004.
2. Blais, J. D. et.al: Activating transcription factor 4 is translationally regulated by hypoxic stress. Molec. Cell. Biol. 24: 7469-7482, 2004.
NCBI and Uniprot Product Information
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Product Notes
The EIF2AK3 (Catalog #AAA73508) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EIF2AK3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the EIF2AK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2AK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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