SDS-PAGE
(The purity of EIF4E protein was determined to be >85% by densitometry. Approx. MW 51 kDa)
EIF4E recombinant protein
EIF4E recombinant protein
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Applications
Western Blot
Purity
>85%
Synonyms
EIF4E; N/A; EIF4E recombinant protein; CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573
Host
E Coli
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Sequence Length
4749
Applicable Applications for EIF4E recombinant protein
WB (Western Blot), Kinase Assay
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length human EIF4E was expressed in E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Stability: 1 yr at -70 degree C from date of shipment.
Shipping: Ships with dry ice.
Stability: 1 yr at -70 degree C from date of shipment.
Shipping: Ships with dry ice.
SDS-PAGE
(The purity of EIF4E protein was determined to be >85% by densitometry. Approx. MW 51 kDa)
SDS-PAGE
(The purity of EIF4E protein was determined to be >85% by densitometry. Approx. MW 51 kDa)
Related Product Information for EIF4E recombinant protein
Recombinant full-length human EIF4E was expressed in E. coli cells using a N-terminal GST tag.
Scientific Background: Eukaryotic Translation Initiation Factor 4E (EIF4E), plays a role in translation initiation where it binds the 5 m7G cap found on mRNAs. EIF4E governs cell cycle progression and cellular proliferation by coordinately the expression of several genes at the post-transcriptional level. EIF4E functions as a central node of an RNA regulon, which plays an essential role in normal differentiation and development (1). The significant association of EIF4E with VEGF and cyclin D1 in multiple tumors supports a role for EIF4E in translational regulation of proteins related to angiogenesis and growth. The overexpression of EIF4E is involved in the malignant progression of human breast cancer (2).
Scientific Background: Eukaryotic Translation Initiation Factor 4E (EIF4E), plays a role in translation initiation where it binds the 5 m7G cap found on mRNAs. EIF4E governs cell cycle progression and cellular proliferation by coordinately the expression of several genes at the post-transcriptional level. EIF4E functions as a central node of an RNA regulon, which plays an essential role in normal differentiation and development (1). The significant association of EIF4E with VEGF and cyclin D1 in multiple tumors supports a role for EIF4E in translational regulation of proteins related to angiogenesis and growth. The overexpression of EIF4E is involved in the malignant progression of human breast cancer (2).
Product Categories/Family for EIF4E recombinant protein
References
1. Culjkovic, B. et al: Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E. Cell Cycle. 2007; 6(1):65-9.
2. Zhou, S. et al: Eukaryotic initiation factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer. BMC Cancer. 2006; 30 (6): 231
2. Zhou, S. et al: Eukaryotic initiation factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer. BMC Cancer. 2006; 30 (6): 231
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~51 kDa
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, mRNA
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
NCBI Protein Information
eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Gene Name
EIF4E
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E
UniProt Entry Name
IF4E_HUMAN
Similar Products
Product Notes
The EIF4E eif4e (Catalog #AAA72907) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||E.coli!!Source Note||Recombinant full-length human EIF4E was expressed in E. coli cells. AAA Biotech's EIF4E can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the EIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.