beta-microseminoprotein ELISA Kit | MSMB elisa kit

Human beta-microseminoprotein ELISA Kit

Cat. Num. AAA87983

• Gene Names: MSMB; MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• Synonyms: beta-microseminoprotein; N/A; Human beta-microseminoprotein ELISA Kit; MSMB elisa kit

• Sequence Length: 114
• Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for MSMB elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This BETA-MICROSEMINOPROTEIN ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of BETA-MICROSEMINOPROTEIN in the sample, this BETA-MICROSEMINOPROTEIN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus BETA-MICROSEMINOPROTEIN concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of BETA-MICROSEMINOPROTEIN utilizes a multiclonal anti-BETA-MICROSEMINOPROTEIN antibody and an BETA-MICROSEMINOPROTEIN-HRP conjugate. The assay sample and buffer are incubated together with BETA-MICROSEMINOPROTEIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BETA-MICROSEMINOPROTEIN concentration since BETA-MICROSEMINOPROTEIN from samples and BETA-MICROSEMINOPROTEIN-HRP conjugate compete for the anti-BETA-MICROSEMINOPROTEIN antibody binding site. Since the number of sites is limited, as more sites are occupied by BETA-MICROSEMINOPROTEIN from the sample, fewer sites are left to bind BETA-MICROSEMINOPROTEIN-HRP conjugate. Standards of known BETA-MICROSEMINOPROTEIN concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of BETA-MICROSEMINOPROTEIN. The BETA-MICROSEMINOPROTEIN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557036
• NCBI GeneID: 4477
• NCBI Accession #: NP_002434.1
• NCBI GenBank Nucleotide #: NM_002443.3
• UniProt Accession #: P08118; P11999; Q13125; Q6IAY9; Q9UC59; B1API6
• Molecular Weight: 12,865 Da
• NCBI Official Full Name: beta-microseminoprotein isoform a
• NCBI Official Synonym Full Names: microseminoprotein, beta-
• NCBI Official Symbol: MSMB
• NCBI Official Synonym Symbols: MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• NCBI Protein Information: beta-microseminoprotein; seminal plasma beta-inhibin; immunoglobulin binding factor; prostate secreted seminal plasma protein; prostate secretory protein of 94 amino acids
• UniProt Protein Name: Beta-microseminoprotein
• UniProt Gene Name: MSMB
• UniProt Synonym Gene Names: PRSP; IGBF; PSP-94; PSP94
• UniProt Entry Name: MSMB_HUMAN