Standard Curve (Sample)
E Coli Host Cell Protein G1 ELISA Kit
E Coli Host Cell Protein ELISA Kit, G1
Synonyms
E Coli Host Cell Protein G1; N/A; E Coli Host Cell Protein ELISA Kit, G1; E Coli Host Cell Protein G1 elisa kit
Samples
Cell Culture Supernatants, Protein Purification Process, and End-Product
Preparation and Storage
Storage at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for E Coli Host Cell Protein G1 elisa kit
Application: The kit is intended for the quantitative determination of residual host cell protein in Cell Culture Supernatants, Protein Purification Process, and End-Product expressed by E. coli expression systems.
Background: Escherichia coli, commonly known as E. coli, is widely utilized as an expression system. During the process of extracting target proteins from fragmented bacterial cells, a substantial portion of host proteins is released along with the target protein. These host proteins exhibit strong immunogenicity, which can lead to adverse toxicity or immune reactions, jeopardizing product safety and quality, and causing potential biological contamination. One of the aims of downstream processes in the production of biological medicinal products is to remove these potential hazards. Therefore, it is essential to minimize the residual levels of host cell proteins (HCP), and in the downstream purification process development, a scientifically sound method for determining the concentration of HCP in finished or semi-finished products is necessary. Enzyme-linked immunosorbent assay (ELISA) has a high sensitivity and is therefore designated as the gold standard for HCP detection by regulatory agencies.
PRINCIPLE OF THE ASSAY This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
Background: Escherichia coli, commonly known as E. coli, is widely utilized as an expression system. During the process of extracting target proteins from fragmented bacterial cells, a substantial portion of host proteins is released along with the target protein. These host proteins exhibit strong immunogenicity, which can lead to adverse toxicity or immune reactions, jeopardizing product safety and quality, and causing potential biological contamination. One of the aims of downstream processes in the production of biological medicinal products is to remove these potential hazards. Therefore, it is essential to minimize the residual levels of host cell proteins (HCP), and in the downstream purification process development, a scientifically sound method for determining the concentration of HCP in finished or semi-finished products is necessary. Enzyme-linked immunosorbent assay (ELISA) has a high sensitivity and is therefore designated as the gold standard for HCP detection by regulatory agencies.
PRINCIPLE OF THE ASSAY This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
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Product Notes
The E Coli Host Cell Protein G1 (Catalog #AAA247881) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "E Coli Host Cell Protein G1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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