Etanercept ELISA Kit
Etanercept ELISA Kit
Synonyms
Etanercept; N/A; Etanercept ELISA Kit; Etanercept elisa kit
Samples
Human Serum And Plasma
Related Product Information for Etanercept elisa kit
Intended Uses: Enzyme Immunoassay for the determination of C-Reactive Protein in human serum and plasma. For research use only.
Principle of the Assay: Microtiterstrips coated with anti-CRP antibody are incubated with diluted standard sera and samples. During this incubation step CRP is bound specifically to the wells. After removal of the unbound serum proteins by a washing procedure, the antigen-antibody complex in each well is detected with specific peroxidase-conjugated antibodies. After removal of the unbound conjugate, the strips are incubated with a chromogen solution containing tetramethylbenzidine and hydrogen peroxide: a blue colour develops in proportion to the amount of immunocomplex bound to the wells of the strips. The enzymatic reaction is stopped by the addition of 0.5M H2S04 and the absorbance values at 450 nm are determined. A standard curve is obtained by plotting the absorbance values versus the corresponding standard values. The concentration of CRP in samples is determined by interpolation from the standard curve.
Principle of the Assay: Microtiterstrips coated with anti-CRP antibody are incubated with diluted standard sera and samples. During this incubation step CRP is bound specifically to the wells. After removal of the unbound serum proteins by a washing procedure, the antigen-antibody complex in each well is detected with specific peroxidase-conjugated antibodies. After removal of the unbound conjugate, the strips are incubated with a chromogen solution containing tetramethylbenzidine and hydrogen peroxide: a blue colour develops in proportion to the amount of immunocomplex bound to the wells of the strips. The enzymatic reaction is stopped by the addition of 0.5M H2S04 and the absorbance values at 450 nm are determined. A standard curve is obtained by plotting the absorbance values versus the corresponding standard values. The concentration of CRP in samples is determined by interpolation from the standard curve.
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Product Notes
The Etanercept (Catalog #AAA72580) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Etanercept, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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