Application Data
Factor XII ELISA Kit | F12 elisa kit
Rat Factor XII total antigen assay ELISA kit
Gene Names
F12; HAF; HAE3; HAEX
Synonyms
Factor XII; N/A; Rat Factor XII total antigen assay ELISA kit; F12 elisa kit
Sequence Length
36
Preparation and Storage
Store at 4 degree C
Application Data
Application Data
Related Product Information for F12 elisa kit
Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation. The sensitive quantitative measurement of total rat Factor XII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XII and XIIa will be detected by the assay. The concentration of Factor XII in normal human plasma ranges from 15-47 ug/ml. Normal values of Factor XII in rat plasma have not been conclusively determined but are believed to be similar to human plasma based on in-house testing. The assay measures total rat Factor XII in the 0.02-10 ng/ml range. Samples giving rat Factor XII levels above 10 ng/ml should be diluted in blocking buffer before use. A 1:10,000 to 1:100,000 dilution for plasma is suggested for best results. Rat Factor XII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XII in the samples. A standard calibration curve is prepared using dilutions of purified Factor XII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Product Categories/Family for F12 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
67,792 Da
NCBI Official Full Name
factor XII, partial
NCBI Official Synonym Full Names
coagulation factor XII
NCBI Official Symbol
F12
NCBI Official Synonym Symbols
HAF; HAE3; HAEX
NCBI Protein Information
coagulation factor XII
UniProt Protein Name
Coagulation factor XII
UniProt Gene Name
F12
UniProt Synonym Gene Names
HAF
UniProt Entry Name
FA12_HUMAN
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Product Notes
The F12 f12 (Catalog #AAA37847) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Factor XII, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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