Folic Acid ELISA Kit

Folic Acid ELISA Kit

Cat. Num. AAA44657

• Synonyms: Folic Acid; N/A; Folic Acid ELISA Kit; Folic Acid elisa kit

• Assay Type: Quantitative Competitive

• Preparation and Storage: Upon receipt, aliquot and store the 100X Folic Acid Conjugate at -20 degree C and avoid multiple freeze/thaw cycles. Store all other components at 4 degree C. The 100X Folic Acid Conjugate and Folic Acid Standard are light sensitive and must be stored accordingly.

Standard Curve (Sample)

Related Product Information for Folic Acid elisa kit

Background: Folic acid is a B vitamin also known as Vitamin B9. Since humans don't synthesize folic acid, it is required from the diet and is therefore considered to be an essential vitamin. In cells, folic acid is required for amino acid metabolism as well as to carry one-carbon groups used for methylation reactions and synthesis of nucleic acids (such as thymine and purine bases). Therefore, deficiency in folic acid disrupts DNA synthesis and cell division, affecting mostly hematopoietic cells and abnormal tissue growths because of their higher rate of cell division. Folic acid is used to supplement folic acid deficiency. This deficiency can cause anemia. Symptoms of anemia can include fatigue, heart palpitations, difficulty breathing, open sores observed on the tongue, and color changes of the hair or skin. Deficiency can occur in children after only a month of consuming a folic acid deficient diet. In adults, normal total body folic acid levels are between 10,000-30,000 micrograms (ug) with plasma levels of greater than 7 nM (3 ng/mL) (Table 1). Women also take supplemental folic acid during pregnancy to prevent fetal neural tube defects (NTDs). Insufficient levels of dietary folic acid in early pregnancy are thought to be the cause of over half of babies born with neural tube defects. As a result, over 50 countries add folic acid to certain foods as a way to decrease NTD incidents in the population. The Folic Acid ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of folic acid in serum, cell or tissue samples. The quantity of folic acid in unknown samples is determined by comparing its absorbance with that of a known folic acid standard curve. The kit has detection sensitivity limit of 24 pg/mL folic acid. Each Folic Acid ELISA Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Principle of the Assay: The Folic Acid ELISA kit is a competitive ELISA for the quantitative measurement of folic acid. The unknown folic acid samples or folic acid standards are first added to a Folic Acid Conjugate preabsorbed microplate. After a brief incubation, an Anti-Folic Acid antibody is added, followed by an HRP conjugated secondary antibody. The folic acid content in unknown samples is determined by comparison with a predetermined folic acid standard curve.

Product Categories/Family for Folic Acid elisa kit

Metabolism Research; Cellular Metabolism; Amino Acid Metabolism