Standard Curve (Sample)
His-Tag Protein ELISA Kit
His-Tag Protein ELISA Kit
Synonyms
His-Tag Protein; His-Tag Protein ELISA Kit; His-Tag Protein elisa kit
Assay Type
Competitive
Preparation and Storage
Store kit components at 4 degree C until their expiration dates.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for His-Tag Protein elisa kit
Background: A polyhistidine-tag, or His-tag, is an amino acid sequence in proteins that consists of at least five histidine (His) residues, usually inserted at the N- or C-terminus of the protein. Often used for affinity purfication of recombinant proteins in E. coli and other prokaryotic or eukaryotic expression systems, the 6xHis-tag motif provides a powerful purification tool while minimizing any effect on the protein's functionality and bioactivity. These attributes make it a popular tag for recombinant protein production and isolation. However, the accurate quantitation of His-tagged protein in unpurified samples remains difficult. His-Tag Protein ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of His-tagged protein samples (C- or N-terminal). The quantity of His-tag in a protein sample is determined by comparing its absorbance with that of a known His-tag protein standard curve (provided in the kit). The kit has detection sensitivity range of 4 ug/mL to 1 ng/mL (10 kDa His-tag protein standard), or 400 nM - 100 pM 6xHis-tag residues. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and His-tag samples. The His-Tag Protein ELISA Kit is intended for research use only, and not for diagnostic applications.
Principle of the Assay: The unknown His-tag samples or recombinant His-tag protein standards are first added to a polyhistidine coated plate. After a brief incubation, an anti-6xHis monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The His-tag protein content in unknown samples is determined by comparing with a standard curve that is prepared from predetermined His-tag protein standards.
Principle of the Assay: The unknown His-tag samples or recombinant His-tag protein standards are first added to a polyhistidine coated plate. After a brief incubation, an anti-6xHis monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The His-tag protein content in unknown samples is determined by comparing with a standard curve that is prepared from predetermined His-tag protein standards.
Product Categories/Family for His-Tag Protein elisa kit
References
1. Porath, J., Carlsson, J., Olsson, I. & Belfrage, G. (1975) Nature 258:598-599.
2. Hemdan, E. S. & Porath, J. (1985) J. Chromatogr. 323:255-264.
3. Hemdan, E. S. & Porath, J. (1985) J. Chromatogr. 323:265-272.
4. Sulkowski, E. (1985) Trends Biotechnol. 3:1-7.
Dong, Y. et al. (2013). HMGB1 Protein Does Not Mediate the Inflammatory Response in Spontaneous Spinal Cord Regeneration: A HINT FOR CNS REGENERATION. J. Biol. Chem. 288:18204-182128.
2. Hemdan, E. S. & Porath, J. (1985) J. Chromatogr. 323:255-264.
3. Hemdan, E. S. & Porath, J. (1985) J. Chromatogr. 323:265-272.
4. Sulkowski, E. (1985) Trends Biotechnol. 3:1-7.
Dong, Y. et al. (2013). HMGB1 Protein Does Not Mediate the Inflammatory Response in Spontaneous Spinal Cord Regeneration: A HINT FOR CNS REGENERATION. J. Biol. Chem. 288:18204-182128.
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Product Notes
The His-Tag Protein (Catalog #AAA11403) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "His-Tag Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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