Standard Curve (Sample)
ICAM-1 / CD54 elisa kit
ICAM-1 / CD54 ELISA Kit
Gene Names
ICAM1; BB2; CD54; P3.58
Synonyms
ICAM-1 / CD54; N/A; ICAM-1 / CD54 ELISA Kit; ICAM-1; Intercellular Adhesion Molecule 1; CD54; Cluster of Differentiation 54; ICAM-1 / CD54 elisa kit
Sequence Length
532
Samples
Mouse Serum, Plasma, Body Fluids, Tissue Homogenate Or Cell Culture Supernatants
Assay Type
Sandwich
Detection Range
15.6 pg/ml-l000 pg/ml
Sensitivity
1 pg /ml
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The %CV of intra-assay is < 10%
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. The %CV of inter-assay is < 10%
Preparation and Storage
2 - 8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ICAM-1 / CD54 elisa kit
Intended Uses: The IBl-America mouse IL-17 ELISA has been designed for the precise analytical measurement of IL-17 concentrations in serum, plasma, body fluids, tissue homogenate or cell culture supernatants from mice. For research use only.
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-wett plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing wi th wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after add ing acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the 0.0,. absorbance at 450nm in a microtiterplale reader, and catcutate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Interleukin-17(IL-17 or IL-17A) originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et ai, in 1993. The IL-7 gene maps to human chromosome 2q31. It is the founding member of a group of cytokines called the IL-17 family, and secreted primarily by activated T cells. IL-17 shows high homology to virallL-17 encoded by an open reading frame of the T-Iymphotropic rhadinovirus Herpesvirus saimiri. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17F has been well characterized both in vitro and in vivo, and has been shown to have a pro-inflammatory role in asthma.
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-wett plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing wi th wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after add ing acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the 0.0,. absorbance at 450nm in a microtiterplale reader, and catcutate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Interleukin-17(IL-17 or IL-17A) originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et ai, in 1993. The IL-7 gene maps to human chromosome 2q31. It is the founding member of a group of cytokines called the IL-17 family, and secreted primarily by activated T cells. IL-17 shows high homology to virallL-17 encoded by an open reading frame of the T-Iymphotropic rhadinovirus Herpesvirus saimiri. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17F has been well characterized both in vitro and in vivo, and has been shown to have a pro-inflammatory role in asthma.
Product Categories/Family for ICAM-1 / CD54 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,825 Da
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
NCBI Protein Information
intercellular adhesion molecule 1
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
UniProt Entry Name
ICAM1_HUMAN
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Product Notes
The ICAM-1 / CD54 icam1 (Catalog #AAA72646) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ICAM-1 / CD54, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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