IGFBP7 elisa kit

IGFBP7 Human ELISA Kit

Cat. Num. AAA59149

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Synonyms: IGFBP7; N/A; IGFBP7 Human ELISA Kit; IGFBP7 elisa kit

• Sequence Length: 282
• Samples: Human serum, body fluids, tissue homogenate or cell culture supernatants
• Assay Type: Quantitative Sandwich
• Detection Range: 625 pg/ml-40,000 pg/ml
• Sensitivity: < 5 pg/ml

• Preparation and Storage: Store at 2-8 degree C for 6 months, or at -20 degree C for 12 months.

Standard Curve (Sample)

Related Product Information for IGFBP7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGFBP7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IGFBP7 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP7 can be calculated.

Background/Introduction: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. Lymphangiogenesis is not only involved in the processes of embryonic development, tissue repair and chronic inflammation, but also in tumor lymphatic metastasis. Metastatic tumor cells spreading through lymphatic vessels occur in non-small cell lung carcinoma (NSCLC), with regional lymph node metastasis often being the most important prognostic factor for carcinoma patients. Insulin-like growth factor binding protein 7 (IGFBP7) was proven to be associated with metastatic clinicopathological features and high LVD. Furthermore, by assessing the capability of lymphatic endothelial cell forming lymphatic vessel-like structures in vitro, it appears to enhance lymphangiogenesis. As a very important transcription factor, signal transducer and activator of transcription 5a (Stat5a) has been reported to be involved in human reproductive cancers such as breast, prostate and ovarian cancer. The mitogenic effect of D5 Stat5a on breast cells is, at least partly, through up-regulation of histone methyltransferase, EZH2, and therefore inhibiting IGFBP-7 expression by increasing H3K27Me3 of IGFBP-7 promoter region.

NCBI and Uniprot Product Information

• NCBI GI #: 23396609
• NCBI GeneID: 3490
• UniProt Accession #: Q16270
• NCBI Official Full Name: Insulin-like growth factor-binding protein 7
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
• UniProt Entry Name: IBP7_HUMAN