Standard Curve (Sample)
Influenza A Nucleoprotein ELISA Kit
Influenza A Nucleoprotein ELISA Kit
Reactivity
Influenza A
Synonyms
Influenza A Nucleoprotein; N/A; Influenza A Nucleoprotein ELISA Kit; Influenza A Nucleoprotein elisa kit
Reactivity
Influenza A
Detection Method
Colorimetric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Influenza A Nucleoprotein elisa kit
Background: The influenza virus is an enveloped virus that can be divided into three classes, A, B, and C, largely based upon conserved antigenic differences in the internal nucleoprotein (NP). Only Influenza A and B are clinically relevant for humans. Influenza A virus, typically encountered more frequently than type B, is associated with the majority of serious epidemics, and it can be further subdivided into strains or subtypes based on antigenic differences in the external hemagglutinin proteins (H1-H16) and neuraminidase proteins (N1-N9). The primary function of NP is to encapsidate the segmented RNA and bind with the three polymerase subunits, PA, PB1 and PB2, to form ribonucleoprotein particles (RNPs) for RNA transcription, replication and packaging. The nfluenza A nucleoprotein ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Influenza A nucleoprotein. The ELISA antibodies only recognize the nucleoprotein from Influenza A, and will not react with the nucleoprotein from Influenza B nor Influenza C. The kit has a detection sensitivity limit of 625 pg/mL Influenza A nucleoprotein. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and Influenza A lysate samples.
Principle of the Assay: An anti-Influenza A nucleoprotein monoclonal antibody is adsorbed onto a microtiter plate. Influenza A nucleoprotein present in the sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-Influenza A nucleoprotein monoclonal antibody is added and binds to the Influenza A nucleoprotein captured by the first antibody. Following incubation and wash steps, an HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-Influenza A nucleoprotein monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of Influenza A nucleoprotein present in the sample. The reaction is terminated by addition of Stop Solution and absorbance is measured at 450 nm. A standard curve is prepared from the provided recombinant Influenza A nucleoprotein standard, and the sample Influenza A nucleoprotein concentration is then determined.
Principle of the Assay: An anti-Influenza A nucleoprotein monoclonal antibody is adsorbed onto a microtiter plate. Influenza A nucleoprotein present in the sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-Influenza A nucleoprotein monoclonal antibody is added and binds to the Influenza A nucleoprotein captured by the first antibody. Following incubation and wash steps, an HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-Influenza A nucleoprotein monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of Influenza A nucleoprotein present in the sample. The reaction is terminated by addition of Stop Solution and absorbance is measured at 450 nm. A standard curve is prepared from the provided recombinant Influenza A nucleoprotein standard, and the sample Influenza A nucleoprotein concentration is then determined.
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Product Notes
The Influenza An Influenza A Nucleoprotein (Catalog #AAA44707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA44707 ELISA Kit recognizes Influenza An Influenza A Nucleoprotein. It is sometimes possible for the material contained within the vial of "Influenza A Nucleoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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