Standard Curve (Sample)
Influenza H1N1 Hemagglutinin/HA ELISA Kit | H1N1 elisa kit
Influenza H1N1 Hemagglutinin/HA ELISA Kit
Reactivity
Influenza H1N1
Synonyms
Influenza H1N1 Hemagglutinin/HA; N/A; Influenza H1N1 Hemagglutinin/HA ELISA Kit; Harvey rat sarcoma viral oncogene homolog; H1N1 elisa kit
Reactivity
Influenza H1N1
Specificity
This assay recognizes most of recombinant H1N1 HA protein. With influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA as the standard, reaction of other H1N1 strains were as follows.
Assay Type
Sandwich
Detection Range
31.25-2000 pg/mL
Sensitivity
14.71 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-assay Precision (Precision between assays) - Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for H1N1 elisa kit
Principle of the Assay: The principle of this ELISA kit is based on the Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The 96-well strip plate is precoated with a monoclonal antibody specific for Influenza H1N1 Hemagglutinin / HA. Standards and Influenza H1N1 Hemagglutinin / HA present in the sample is bound by the immobilized antibody. After washing, a horseradish peroxidase conjugated anti-Influenza H1N1 Hemagglutinin / HA antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody -enzyme reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Influenza H1N1 Hemagglutinin / HA bound in the initial step. The color development is stopped and the intensity of the color is measured at 450 nm.
Intended Uses: The kit has been verified by high purity Influenza H1N1 Hemagglutinin / HA recombinant protein. The use of this kit for natural samples need to be validated by the end user due to the complexity of natural targets and unpredictable interference.
Intended Uses: The kit has been verified by high purity Influenza H1N1 Hemagglutinin / HA recombinant protein. The use of this kit for natural samples need to be validated by the end user due to the complexity of natural targets and unpredictable interference.
References
White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018
Similar Products
Product Notes
The Influenza H1N1 H1N1 (Catalog #AAA258415) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258415 ELISA Kit recognizes Influenza H1N1 H1N1. It is sometimes possible for the material contained within the vial of "Influenza H1N1 Hemagglutinin/HA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.