Influenza H3N2 Hemagglutinin/HA ELISA Kit | H3N2 elisa kit

Influenza H3N2 Hemagglutinin/HA ELISA Kit

Cat. Num. AAA258416

• Reactivity: Influenza H3N2
• Synonyms: Influenza H3N2 Hemagglutinin/HA; N/A; Influenza H3N2 Hemagglutinin/HA ELISA Kit; Harvey rat sarcoma viral oncogene homolog; H3N2 elisa kit

• Reactivity: Influenza H3N2
• Specificity: This assay recognizes most of recombinant H3N2 HA proteins. Influenza A H3N2 (A/Brisbane/10/2007) hemagglutinin / HA was served as the reference standard, and reaction of other H3N2 strains were as follows.
• Assay Type: Sandwich
• Sensitivity: < 263.49 pg/mL

• Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision: Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Related Product Information for H3N2 elisa kit

Background: Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. Among the multiple influenza A virus subtypes, H3N2 is one of the three subtypes which mainly infects humans. H3N2 viruses can infect birds, humans, and pigs, and the virus has mutated into many strains.

Principle of the Assay: The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for Influenza H3N2 Hemagglutinin / HA has been pre-coated onto well plate strips. Standards and samples are added to the wells and Influenza H3N2 Hemagglutinin / HA present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-Influenza H3N2 Hemagglutinin / HA antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of Influenza H3N2 Hemagglutinin / HA bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).

Intended Uses: The kit has been verified by high purity Influenza H3N2 Hemagglutinin / HA recombinant protein.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018