Application Data
Kininogen ELISA Kit | KNG1 elisa kit
Human Kininogen total antigen assay ELISA kit
Gene Names
KNG1; BK; BDK; KNG
Synonyms
Kininogen; N/A; Human Kininogen total antigen assay ELISA kit; KNG1 elisa kit
Sequence Length
427
Preparation and Storage
Store at 4 degree C
Application Data
Application Data
Related Product Information for KNG1 elisa kit
High molecular weight kininogen (HK, aka Fitzgerald Factor) is the single chain 626 amino acid 120kDa glycoprotein precursor of the vasoactive peptide bradykinin. Kininogen is an important cofactor for the activation of zymogens prekallikrein, Factor XII, and Factor XI in the contact activation or intrinsic coagulation pathway. Additionally kininogen is a major inhibitor of systemic cysteine proteinases such cathepsins, calpain and papain. The sensitive quantitative measurement of total human kininogen antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. The concentration of kininogen in normal human plasma is 65-115 ug/ml with an average concentration of 83 ug/ml. The assay measures human kininogen in the 0.07-70 ng/ml range. Samples giving human kininogen levels above 70 ug/ml should be diluted in blocking buffer before use. A 1:10,000-1:50,000 dilution for plasma is suggested for best results. Human kininogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase conjugated polyclonal anti-human kininogen primary antibody binds to the captured protein. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of kininogen in the samples. A standard calibration curve is prepared using dilutions of purified kininogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Product Categories/Family for KNG1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,822 Da
NCBI Official Full Name
kininogen
NCBI Official Synonym Full Names
kininogen 1
NCBI Official Symbol
KNG1
NCBI Official Synonym Symbols
BK; BDK; KNG
NCBI Protein Information
kininogen-1
UniProt Protein Name
Kininogen-1
UniProt Gene Name
KNG1
UniProt Synonym Gene Names
BDK; KNG; HMWK
UniProt Entry Name
KNG1_HUMAN
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Product Notes
The KNG1 kng1 (Catalog #AAA37853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Kininogen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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