Standard Curve (Sample)
Listeria monocytogenes p60 ELISA Kit
Listeria monocytogenes p60 ELISA Kit
Reactivity
Listeria
Synonyms
Listeria monocytogenes p60; N/A; Listeria monocytogenes p60 ELISA Kit; Listeria monocytogenes p60 elisa kit
Reactivity
Listeria
Detection Method
Colorimetric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Listeria monocytogenes p60 elisa kit
Background: Listeriosis is a serious infection caused by the germ Listeria monocytogenes. People usually become ill with listeriosis after eating contaminated food. The disease primarily affects pregnant women, newborns, older adults, and people with weakened immune systems. The initial internalization of Listeria monocytogenes in the mammalian cells occurs through the surface proteins internalin (InlA and InlB). However, the pore?forming protein listeriolysin O (LLO) is the primary virulence factor of Listeria monocytogenes, as it helps the bacteria to escape phagosome of host cells and further multiplication inside the host cytoplasm. The p60 protein encoded by the iap (invasion-associated protein) gene is expressed on the cell surface, but it is mainly secreted in large quantities in the culture supernatant of Listeria monocytogenes. This makes the p60 protein an ideal indicator protein for the detection of Listeria monocytogenes from contaminated foods. The Listeria monocytogenes p60 ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of the Listeria monocytogenes p60 protein. The ELISA monoclonal antibodies specifically recognize the p60 protein secreted from Listeria monocytogenes and will not react with other food borne bacteria such as E. coli O157, Salmonella, and Shigella. The ELISA kit has a detection sensitivity limit of 625 pg/mL Listeria monocytogenes p60. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and food test samples.
Principle of the Assay: An anti-Listeria monocytogenes p60 monoclonal antibody is adsorbed onto a microtiter plate. Listeria monocytogenes p60 present in the pre-enriched sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-Listeria monocytogenes p60 monoclonal antibody is added and binds to the Listeria monocytogenes p60 captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-Listeria monocytogenes p60 monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and Substrate Solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of Listeria monocytogenes p60 present in the sample. The reaction is terminated by addition of Stop Solution and the absorbance is measured at 450 nm. A standard curve is prepared from the provided recombinant Listeria monocytogenes p60 standard and the sample Listeria monocytogenes p60 amount is then determined.
Principle of the Assay: An anti-Listeria monocytogenes p60 monoclonal antibody is adsorbed onto a microtiter plate. Listeria monocytogenes p60 present in the pre-enriched sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-Listeria monocytogenes p60 monoclonal antibody is added and binds to the Listeria monocytogenes p60 captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-Listeria monocytogenes p60 monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and Substrate Solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of Listeria monocytogenes p60 present in the sample. The reaction is terminated by addition of Stop Solution and the absorbance is measured at 450 nm. A standard curve is prepared from the provided recombinant Listeria monocytogenes p60 standard and the sample Listeria monocytogenes p60 amount is then determined.
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Product Notes
The Listeria Listeria monocytogenes p60 (Catalog #AAA44701) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA44701 ELISA Kit recognizes Listeria Listeria monocytogenes p60. It is sometimes possible for the material contained within the vial of "Listeria monocytogenes p60, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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