Standard Curve (Sample)
OxiSelect Advanced Glycation End Product (AGE) ELISA Kit
OxiSelect Advanced Glycation End Product (AGE) Competitive ELISA Kit
Synonyms
OxiSelect Advanced Glycation End Product (AGE); N/A; OxiSelect Advanced Glycation End Product (AGE) Competitive ELISA Kit; OxiSelect Advanced Glycation End Product (AGE) elisa kit
Preparation and Storage
Upon receipt, aliquot and store the Anti-AGE Antibody, AGE-BSA Standard, AGE Conjugate and 100X Conjugate Diluent at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for OxiSelect Advanced Glycation End Product (AGE) elisa kit
Principle of the Assay: First, an AGE conjugate is coated on an ELISA plate. The unknown AGE protein samples or AGE-BSA standards are then added to the AGE conjugate preabsorbed ELISA plate. After a brief incubation, an anti-AGE polyclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of AGE protein adducts in unknown samples is determined by comparison with a predetermined AGE-BSA standard curve.!!Background/Introduction: The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. For example, N-epsilon-(Carboxymethyl) lysine (CML), one of the prevalent AGEs, has been implicated in oxidative stress and vascular damage. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events. OxiSelect™ AGE Competitive ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of AGE protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its absorbance with that of a known AGE-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Product Categories/Family for OxiSelect Advanced Glycation End Product (AGE) elisa kit
References
1. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.
2. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9468.
3. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
4. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
5. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
Foster, D. et al. (2014). AGE Metabolites: A Biomarker Linked to Cancer Disparity? Cancer Epidemiol Biomarkers Prev. 23:2186-2191.
2. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9468.
3. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.
4. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.
5. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.
Foster, D. et al. (2014). AGE Metabolites: A Biomarker Linked to Cancer Disparity? Cancer Epidemiol Biomarkers Prev. 23:2186-2191.
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Product Notes
The OxiSelect Advanced Glycation End Product (AGE) (Catalog #AAA11421) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect Advanced Glycation End Product (AGE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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