Standard Curve (Sample)
SuperNuclease ELISA Kit
SuperNuclease ELISA Kit
Synonyms
SuperNuclease; N/A; SuperNuclease ELISA Kit; SuperNuclease elisa kit
Assay Type
Quantitative Sandwich
Sensitivity
< 14.55 pg/mL
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Preparation and Storage
Unopened Kit: Store at 2-8 degree C Opened/Reconstituted Reagents: Please refer to CoA
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SuperNuclease elisa kit
Background: The SuperNuclease is a nonspecific nuclease with high activity, capable of completely digesting RNA and DNA (single stranded, double stranded, linear, circular and super coiled forms, that no fewer than five phosphate residues [1]) into 5'-monophosphate-terminated oligonucleotides of 3-5 bases in length [2]. SuperNuclease requires divalent cation, preferably Mg2+ for activity, displays a broad pH tolerance (range from 6 to 10, optimal at 8-8.5) and has a wide temperature optimum between 35? and 44? [3]. The nuclease is a homodimer (the dimer form is physiologic and functions more progressively than the monomer [3,4,5,6,7]). Two disulfide bonds in the nuclease are crucial to its activity and stability [9]. It does not have typical protease activity detected by azocasein assay. Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological and pharmaceutical applications. SuperNuclease can be removed by various purification methods.
Principle of the Assay: The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for SuperNuclease has been pre-coated onto well plate strips. Standards and samples are added to the wells and SuperNuclease present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-SuperNuclease antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of SuperNuclease bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).
Intended Uses: The kit is used for detection and quantitative determination of SuperNuclease and Benzonase endonuclease, and also can detect the denatured SuperNuclease and Benzonase.
Principle of the Assay: The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for SuperNuclease has been pre-coated onto well plate strips. Standards and samples are added to the wells and SuperNuclease present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-SuperNuclease antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of SuperNuclease bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).
Intended Uses: The kit is used for detection and quantitative determination of SuperNuclease and Benzonase endonuclease, and also can detect the denatured SuperNuclease and Benzonase.
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Product Notes
The SuperNuclease (Catalog #AAA258578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SuperNuclease, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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