Standard Curve (Sample)
Canine Epithelial Cell Adhesion Molecule ELISA Kit | EPCAM elisa kit
Canine Epithelial Cell Adhesion Molecule ELISA Kit
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Canine
Synonyms
Epithelial Cell Adhesion Molecule; N/A; Canine Epithelial Cell Adhesion Molecule ELISA Kit; EPCAM elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
5.0-100 U/mL
Sensitivity
1.0u/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for EPCAM elisa kit
Intended Uses: This EPCAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine EPCAM. This ELISA kit for research use only!
Principle of the Assay: EPCAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPCAM antibody and an EPCAM-HRP conjugate. The assay sample and buffer are incubated together with EPCAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPCAM concentration since EPCAM from samples and EPCAM-HRP conjugate compete for the anti-EPCAM antibody binding site. Since the number of sites is limited, as more sites are occupied by EPCAM from the sample, fewer sites are left to bind EPCAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPCAM concentration in each sample is interpolated from this standard curve.
Principle of the Assay: EPCAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPCAM antibody and an EPCAM-HRP conjugate. The assay sample and buffer are incubated together with EPCAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPCAM concentration since EPCAM from samples and EPCAM-HRP conjugate compete for the anti-EPCAM antibody binding site. Since the number of sites is limited, as more sites are occupied by EPCAM from the sample, fewer sites are left to bind EPCAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPCAM concentration in each sample is interpolated from this standard curve.
Product Categories/Family for EPCAM elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,932 Da
NCBI Official Full Name
Epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein)
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
UniProt Entry Name
EPCAM_HUMAN
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Product Notes
The Canine EPCAM epcam (Catalog #AAA92545) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA92545 ELISA Kit recognizes Canine EPCAM. It is sometimes possible for the material contained within the vial of "Epithelial Cell Adhesion Molecule, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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