ERBB4 recombinant protein

ErbB4 Protein, Human, Rhesus, Recombinant (His Tag)

Cat. Num. AAA257418

• Gene Names: ERBB4; HER4; ALS19; p180erbB4
• Purity: >85 % as determined by SDS-PAGE
• Synonyms: ErbB4; N/A; ErbB4 Protein, Human, Rhesus, Recombinant (His Tag); Human/Rhesus HER4/ErbB4 Protein (His Tag); erb-b2 receptor tyrosine kinase 4; ERBB4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 635aa
• Sequence: Met1-Arg649

• Species: Human, Rhesus
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln26
• Protein Construction: |A DNA sequence encoding the human ERBB4 (NP_005226.1) (Met1-Arg649) with a C-terminal polyhistidine tag was expressed. Human and Rhesus ERBB4 sequences are identical
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human ErbB4-His at 10ug/ml (100ul/well) can bind biotinylated human NRG1 (Cat:11609-H01H2). The EC50 of biotinylated human NRG1 (Cat:11609-H01H2) is 0.4-0.92ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human ErbB4-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1 (Cat: 11609-H01H2), The EC50 of biotinylated human NRG1 (Cat: 11609-H01H2) is 0.4-0.92 ug/ml.)

Application Data

Related Product Information for ERBB4 recombinant protein

ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

Product Categories/Family for ERBB4 recombinant protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Huang, Y Z, et al. (2000) Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses. Neuron. 26(2):443-55.
Garcia, R A, et al. (2000) The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses. Proc Natl Acad Sci. 97(7):3596-601.
Silberberg G, et al. (2006) The involvement of ErbB4 with schizophrenia: association and expression studies. Am J Med Genet. 141(B2):142-8.
Sardi SP, et al. (2006) Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell. 127(1):185-97.

NCBI and Uniprot Product Information

• NCBI GI #: 4885215
• NCBI GeneID: 2066
• NCBI Accession #: NP_005226.1
• NCBI GenBank Nucleotide #: NM_005235.2
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 143,968 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; ALS19; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; human epidermal growth factor receptor 4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblast
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN