Human Epiregulin ELISA Kit | EREG elisa kit

Human Epiregulin ELISA Kit

Cat. Num. AAA263041

• Gene Names: EREG; ER; Ep; EPR
• Reactivity: Human
• Synonyms: Epiregulin; N/A; Human Epiregulin ELISA Kit; Proepiregulin; EREG elisa kit

• Reactivity: Human
• Specificity: The Human Epiregulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Epiregulin proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 169
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for EREG elisa kit

Principle of the Assay: The Human Epiregulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Epiregulin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Epiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 4557567
• NCBI GeneID: 2069
• NCBI Accession #: NP_001423.1
• NCBI GenBank Nucleotide #: NM_001432.2
• UniProt Accession #: O14944; Q6FH69; B2RC66
• Molecular Weight: 19,044 Da
• NCBI Official Full Name: proepiregulin preproprotein
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: EREG
• NCBI Official Synonym Symbols: ER; Ep; EPR
• NCBI Protein Information: proepiregulin
• UniProt Protein Name: Proepiregulin
• UniProt Gene Name: EREG
• UniProt Synonym Gene Names: EPR
• UniProt Entry Name: EREG_HUMAN