Mouse Erythroferrone ELISA Kit | ERFE elisa kit

Mouse Erythroferrone ELISA Kit

Cat. Num. AAA27427

• Gene Names: ERFE; CTRP15; FAM132B; C1QTNF15
• Reactivity: Mouse
• Synonyms: Erythroferrone; N/A; Mouse Erythroferrone ELISA Kit; ERFE elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ERFE. No significant cross-reactivity or interference between ERFE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ERFE and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 354
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for ERFE elisa kit

Principle of the Assay: ERFE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ERFE antibody and an ERFE-HRP conjugate. The assay sample and buffer are incubated together with ERFE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ERFE concentration since ERFE from samples and ERFE-HRP conjugate compete for the anti-ERFE antibody binding site. Since the number of sites is limited, as more sites are occupied by ERFE from the sample, fewer sites are left to bind ERFE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERFE concentration in each sample is interpolated from this standard curve.

Intended Uses: This ERFE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ERFE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 628601881
• NCBI GeneID: 151176
• NCBI Accession #: NP_001278761.1
• NCBI GenBank Nucleotide #: NM_001291832.2
• UniProt Accession #: Q4G0M1; W8S2M9
• Molecular Weight: 37,279 Da
• NCBI Official Full Name: erythroferrone
• NCBI Official Synonym Full Names: erythroferrone
• NCBI Official Symbol: ERFE
• NCBI Official Synonym Symbols: CTRP15; FAM132B; C1QTNF15
• NCBI Protein Information: erythroferrone
• UniProt Protein Name: Erythroferrone
• UniProt Gene Name: ERFE
• UniProt Synonym Gene Names: C1QTNF15; CTRP15; FAM132B