Bovine Extracellular Signal Regulated Kinase ELISA Kit | ERK elisa kit

Bovine Extracellular Signal Regulated Kinase ELISA Kit

Cat. Num. AAA97491

• Gene Names: MAPK12; ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA
• Reactivity: Bovine
• Synonyms: Extracellular Signal Regulated Kinase; N/A; Bovine Extracellular Signal Regulated Kinase ELISA Kit; ERK elisa kit

• Reactivity: Bovine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive

• Intended Uses: This ERK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ERK. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This ERK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ERK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ERK elisa kit

Principle of the Assay: ERK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ERK antibody and an ERK-HRP conjugate. The assay sample and buffer are incubated together with ERK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ERK concentration since ERK from samples and ERK-HRP conjugate compete for the anti-ERK antibody binding site. Since the number of sites is limited, as more sites are occupied by ERK from the sample, fewer sites are left to bind ERK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERK concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ERK elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1486363
• NCBI GeneID: 6300
• UniProt Accession #: P53778; Q14260; Q6IC53; Q99588; Q99672
• Molecular Weight: 40,808 Da
• NCBI Official Full Name: extracellular signal regulated kinase
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 12
• NCBI Official Symbol: MAPK12
• NCBI Official Synonym Symbols: ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA
• NCBI Protein Information: mitogen-activated protein kinase 12; ERK-6; MAPK 12; MAP kinase 12; MAP kinase p38 gamma; stress-activated protein kinase 3; mitogen-activated protein kinase 3; extracellular signal-regulated kinase 6; mitogen-activated protein kinase p38 gamma
• UniProt Protein Name: Mitogen-activated protein kinase 12
• UniProt Gene Name: MAPK12
• UniProt Synonym Gene Names: ERK6; SAPK3; MAP kinase 12; MAPK 12; ERK-6; MAP kinase p38 gamma
• UniProt Entry Name: MK12_HUMAN