Application Data
(Recombinant EZH1 complex HTRF activity assay 3.3 ?M H3K27me0 (16-37aa) peptide was incubated with EZH1 complex in reaction buffer for 3 hour at room temperature. EZH1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me1) was measured using H3K27me1-specific antibody.)
EZH1 recombinant protein
Recombinant EZH1 complex
Gene Names
EZH1; KMT6B
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Synonyms
EZH1; N/A; Recombinant EZH1 complex; Enhancer Of Zeste 1; EC 2.1.1.43; EZH1 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >95% pure by SDS-PAGE.
Form/Format
Recombinant EZH1 complex was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant EZH1 complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 3.3uM H3K27me0 (16-37aa) peptide was incubated with different concentrations of recombinant EZH1 complex in reaction buffer containing 50mM TrisCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM SAM for 3 hours at room temperature. Activity was detected by HTRF.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant EZH1 complex HTRF activity assay 3.3 ?M H3K27me0 (16-37aa) peptide was incubated with EZH1 complex in reaction buffer for 3 hour at room temperature. EZH1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me1) was measured using H3K27me1-specific antibody.)
Application Data
(Recombinant EZH1 complex HTRF activity assay 3.3 ?M H3K27me0 (16-37aa) peptide was incubated with EZH1 complex in reaction buffer for 3 hour at room temperature. EZH1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me1) was measured using H3K27me1-specific antibody.)
SDS-PAGE
(Recombinant EZH1 complex gel EZH1 complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant EZH1 complex gel EZH1 complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for EZH1 recombinant protein
Short Description: Recombinant EZH1 complex contains full-length human EZH1 protein (accession number NP_001982.2) with N-terminal DYKDDDDK-Tag and MW = 86.3 kDa; full-length human SUZ12 (Accession No. NP_056170.2), with N-terminal 6xHis-tag and MW = 86.1 kDa; full-length human EED (Accession No. NP_003788.2), N-terminal 6xHis-tag and MW = 53.2 kDa; full-length human RbAp46 (Accession No. NP_001185648.1), N-terminal 6xHis-tag and MW = 55.3 kDa, and full-length human RbAp48 (Accession No. NP_005601.1), N-terminal 6xHis-tag and MW = 50.7 kDa, all individually expressed in Sf9 cells. The recombinant protein is >=95% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: EZH1 (Enhancer Of Zeste Homolog 1) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Background: EZH1 (Enhancer Of Zeste Homolog 1) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Product Categories/Family for EZH1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,271 Da
NCBI Official Full Name
histone-lysine N-methyltransferase EZH1
NCBI Official Synonym Full Names
enhancer of zeste homolog 1 (Drosophila)
NCBI Official Symbol
EZH1
NCBI Official Synonym Symbols
KMT6B
NCBI Protein Information
histone-lysine N-methyltransferase EZH1; ENX-2
UniProt Protein Name
Histone-lysine N-methyltransferase EZH1
UniProt Gene Name
EZH1
UniProt Synonym Gene Names
KIAA0388
UniProt Entry Name
EZH1_HUMAN
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Product Notes
The EZH1 ezh1 (Catalog #AAA59721) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EZH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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