Human coagulation factor X ELISA Kit | F10 elisa kit

Human coagulation factor X, FX ELISA Kit

Cat. Num. AAA79842

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Synonyms: coagulation factor X; N/A; Human coagulation factor X, FX ELISA Kit; Human coagulation factor X; FX ELISA Kit; RP11-98F14.9; FX; FXA; Stuart-Prower factor; factor Xa; prothrombinase; F10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human F10. No significant cross-reactivity or interference between Human F10 and analogues was observed.
• Sequence Length: 488

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for F10 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for FX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor
• UniProt Protein Name: Coagulation factor X
• UniProt Gene Name: F10
• UniProt Entry Name: FA10_HUMAN