Human Coagulation Factor XIII B Polypeptide ELISA Kit | F13B elisa kit

Human Coagulation Factor XIII B Polypeptide ELISA Kit

Cat. Num. AAA86731

• Gene Names: F13B; FXIIIB
• Reactivity: Human
• Synonyms: Coagulation Factor XIII B Polypeptide; N/A; Human Coagulation Factor XIII B Polypeptide ELISA Kit; F13B elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for F13B elisa kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This F13B ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of F13B in the sample, this F13B ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus F13B concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of F13B utilizes a multiclonal anti-F13B antibody and an F13B-HRP conjugate. The assay sample and buffer are incubated together with F13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F13B concentration since F13B from samples and F13B-HRP conjugate compete for the anti-F13B antibody binding site. Since the number of sites is limited, as more sites are occupied by F13B from the sample, fewer sites are left to bind F13B-HRP conjugate. Standards of known F13B concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of F13B. The F13B concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110611237
• NCBI GeneID: 2165
• NCBI Accession #: NP_001985.2
• NCBI GenBank Nucleotide #: NM_001994.2
• Molecular Weight: 75,511 Da
• NCBI Official Full Name: coagulation factor XIII B chain
• NCBI Official Synonym Full Names: coagulation factor XIII, B polypeptide
• NCBI Official Symbol: F13B
• NCBI Official Synonym Symbols: FXIIIB
• NCBI Protein Information: coagulation factor XIII B chain; TGase; transglutaminase B chain; fibrin-stabilizing factor B subunit; protein-glutamine gamma-glutamyltransferase B chain
• UniProt Protein Name: Coagulation factor XIII B chain
• UniProt Gene Name: F13B
• UniProt Entry Name: F13B_HUMAN