SDS-PAGE
Coagulation Factor VIII (F8) Recombinant Protein | F8 recombinant protein
Recombinant Coagulation Factor VIII (F8)
Gene Names
F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E
Applications
Western Blot, SDS-Page
Purity
> 90%
Synonyms
Coagulation Factor VIII (F8); N/A; Recombinant Coagulation Factor VIII (F8); F8 recombinant protein
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 100ug/mL (varies by lot)
Sequence
GRQV TVQEFALFFT IFDETKSWYF TENMERNCRA PCNIQMEDPT FKENYRFHAI NGYIMDTLPG LVMAQDQRIR WYLLSMGSNE NIHSIHFSGH VFTVRKKEEYKMALYNLYPG VFETVEMLPS KAGIWRVECL IGEHLHAGMS TLFLVYSNKC QTPL
Applicable Applications for F8 recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Gly1887~Leu2044
Tags
N-terminal His Tag
Subcellular Location
Secreted, Extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
6.4
Usage
Reconstitute in ddH2O to a concentration of 0-0.2mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaws cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The lost rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The lost rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.8kDa
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
coagulation factor VIII isoform a
NCBI Official Synonym Full Names
coagulation factor VIII, procoagulant component
NCBI Official Symbol
F8
NCBI Official Synonym Symbols
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
NCBI Protein Information
coagulation factor VIII; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc
UniProt Protein Name
Coagulation factor VIII
UniProt Gene Name
F8
UniProt Synonym Gene Names
F8C; AHF
UniProt Entry Name
FA8_HUMAN
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Product Notes
The F8 f8 (Catalog #AAA134293) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Coagulation Factor VIII (F8) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the F8 f8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GRQV TVQEFALFFT IFDETKSWYF TENMERNCRA PCNIQMEDPT FKENYRFHAI NGYIMDTLPG LVMAQDQRIR WYLLSMGSNE NIHSIHFSGH VFTVRKKEEY KMALYNLYPG VFETVEMLPS KAGIWRVECL IGEHLHAGMS TLFLVYSNKC QTPL. It is sometimes possible for the material contained within the vial of "Coagulation Factor VIII (F8), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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