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product-image-AAA92674_SC15.jpg Standard Curve (Sample)

Chicken Coagulation Factor IX ELISA Kit | F9 elisa kit

Chicken Coagulation Factor IX ELISA Kit

Gene Names
F9; FIX; P19; PTC; HEMB; THPH8
Reactivity
Chicken
Synonyms
Coagulation Factor IX; N/A; Chicken Coagulation Factor IX ELISA Kit; F9 elisa kit
Ordering
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This F9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken F9. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C

Standard Curve (Sample)

product-image-AAA92674_SC15.jpg Standard Curve (Sample)
Related Product Information for F9 elisa kit
Principle of the Assay: F9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F9 antibody and an F9-HRP conjugate. The assay sample and buffer are incubated together with F9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F9 concentration since F9 from samples and F9-HRP conjugate compete for the anti-F9 antibody binding site. Since the number of sites is limited, as more sites are occupied by F9 from the sample, fewer sites are left to bind F9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F9 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for F9 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
51,778 Da
NCBI Official Full Name
coagulation factor IX
NCBI Official Synonym Full Names
coagulation factor IX
NCBI Official Symbol
F9
NCBI Official Synonym Symbols
FIX; P19; PTC; HEMB; THPH8
NCBI Protein Information
coagulation factor IX; F9 p22; FIX F9; factor 9; factor IX F9; serine protease; Christmas factor; plasma thromboplastic component; plasma thromboplastin component
UniProt Protein Name
Coagulation factor IX
UniProt Gene Name
F9
UniProt Synonym Gene Names
PTC
UniProt Entry Name
FA9_HUMAN

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Product Notes

The Chicken F9 f9 (Catalog #AAA92674) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA92674 ELISA Kit recognizes Chicken F9. It is sometimes possible for the material contained within the vial of "Coagulation Factor IX, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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