CD64 Recombinant Protein | FCGR1 recombinant protein

CD64 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA257467

• Gene Names: Fcgr1; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
• Purity: >97 % as determined by SDS-PAGE
• Synonyms: CD64; N/A; CD64 Protein, Mouse, Recombinant (His Tag); Mouse CD64/FCGR1 Protein (His Tag); Fc receptor, IgG, high affinity I; AI323638 Protein, Mouse; AV092959 Protein, Mouse; CD64 Protein, Mouse; FcgammaRI Protein, Mouse; IGGHAFC Protein, Mouse; FCGR1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 284aa
• Sequence: Met1-Pro297

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu25
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) precursor was fused with a polyhistidine tag at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse CD64-His at 10ug/ml (100ul/well) can bind biotinylated human IgG1. The EC50 of biotinylated human IgG1 is 0.07-0.17ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA.  Immobilized mouse CD64-His at 10 ug/ml (100 ul/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 ug/ml.)

Application Data

Related Product Information for FCGR1 recombinant protein

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNgammaandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Product Categories/Family for FCGR1 recombinant protein

Fc Receptors

References

Allen J.M., et al., (1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.Porges A.J., et al., (1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.

NCBI and Uniprot Product Information

• NCBI GI #: 6753832
• NCBI GeneID: 14129
• NCBI Accession #: NP_034316.1
• NCBI GenBank Nucleotide #: NM_010186.5
• UniProt Accession #: P26151
• Molecular Weight: 44,888 Da
• NCBI Official Full Name: high affinity immunoglobulin gamma Fc receptor I
• NCBI Official Synonym Full Names: Fc receptor, IgG, high affinity I
• NCBI Official Symbol: Fcgr1
• NCBI Official Synonym Symbols: CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
• NCBI Protein Information: high affinity immunoglobulin gamma Fc receptor I
• UniProt Protein Name: High affinity immunoglobulin gamma Fc receptor I
• UniProt Gene Name: Fcgr1
• UniProt Synonym Gene Names: Fcg1; IgG Fc receptor I; FcRI
• UniProt Entry Name: FCGR1_MOUSE