Canine Fibroblast Growth Factor 21 ELISA Kit | FGF-21 elisa kit
Canine Fibroblast Growth Factor 21 ELISA Kit
Reactivity
Canine
Synonyms
Fibroblast Growth Factor 21; N/A; Canine Fibroblast Growth Factor 21 ELISA Kit; FGF-21 elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of FGF-21. No significant cross-reactivity or interference between FGF-21 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FGF-21 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for FGF-21 elisa kit
Intended Uses: This FGF-21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of CanineFGF-21. This ELISA kit for research use only!
Principle of the Assay: FGF-21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FGF-21 antibody and an FGF-21-HRP conjugate. The assay sample and buffer are incubated together with FGF-21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-21 concentration since FGF-21 from samples and FGF-21-HRP conjugate compete for the anti-FGF-21 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-21 from the sample, fewer sites are left to bind FGF-21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-21 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FGF-21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FGF-21 antibody and an FGF-21-HRP conjugate. The assay sample and buffer are incubated together with FGF-21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-21 concentration since FGF-21 from samples and FGF-21-HRP conjugate compete for the anti-FGF-21 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-21 from the sample, fewer sites are left to bind FGF-21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-21 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FGF-21 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,300 Da
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
UniProt Protein Name
Fibroblast growth factor 21
UniProt Gene Name
FGF21
UniProt Synonym Gene Names
UNQ3115/PRO10196; FGF-21
UniProt Entry Name
FGF21_HUMAN
