FGF-8b recombinant protein

Recombinant human FGF-8b protein

Cat. Num. AAA48424

• Gene Names: FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: FGF-8b; N/A; Recombinant human FGF-8b protein; FGF8; AIGF; Androgen-induced growth factor; FGF-8; HBGF-8; Heparin-binding growth factor 8; HH6; KAL6;fibroblast growth factor 8; fibroblast growth factor 8 isoform B; FGF-8b recombinant protein

• Host: HEK293
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 23-215aa
• Sequence: QVTVQ SSPNFTQHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR

• Applicable Applications for FGF-8b recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: His-Tag
• Predicted Nterm: Asp
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 ug/ml of heparin. The ED50 range <= 5 ug/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human FGF-8b stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 ug/ml of heparin. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for FGF-8b recombinant protein

FGF-8b, also known as Fibroblast growth factor 8 isoform B, is a member of the fibroblast growth factor (FGF) family that plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF-8; FGF-8a, FGF-8b, FGF-8e and FGF-8f. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It encodes a key signaling factor, and its precise regulation is essential for embryo patterning. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity. FGF-8 signaling from the apical ectodermal ridge (AER), which borders the distal end of the limb bud, is necessary for forming normal limbs. FGF-8 is responsible for cell proliferation and survival, as well. The ability for FGF-8 to regulate cell proliferation has caused interest in its effects on tumors or squamous cell carcinoma. Recombinant human FGF-8b, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for FGF-8b recombinant protein

Angiogenesis; Cardiovascular

References

Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928-8932.;; Ornitz DM, et al. (1996). The Journal of Biological Chemistry. 271: 15292-15297.;; Crossley, P.H. et al. (1996) Cell, 84:127-136.;; Estienne A, et al.(2018) Reproduction. 155:53-62.;;

NCBI and Uniprot Product Information

• NCBI GI #: 5174439
• NCBI GeneID: 2253
• NCBI Accession #: NP_006110.1
• NCBI GenBank Nucleotide #: NM_006119.4
• UniProt Accession #: P55075; Q14915; Q15766; A1A514
• Molecular Weight: 26,525 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform B
• NCBI Official Synonym Full Names: fibroblast growth factor 8 (androgen-induced)
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: HH6; AIGF; KAL6; FGF-8; HBGF-8
• NCBI Protein Information: fibroblast growth factor 8; androgen-induced growth factor; heparin-binding growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: FGF8
• UniProt Synonym Gene Names: AIGF; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_HUMAN