FGFBP3 recombinant protein

FGFBP3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257957

• Gene Names: FGFBP3; FGF-BP3; C10orf13
• Purity: >87% as determined by SDS-PAGE
• Synonyms: FGFBP3; N/A; FGFBP3 Protein, Human, Recombinant (His Tag); Human FGFBP3 Protein (His Tag); C10orf13 Protein, Human; FGF-BP3 Protein, Human; fibroblast growth factor binding protein 3; FGFBP3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >87% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly258

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FGFBP3 (Met 1-Gly 258)(Q8TAT2) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-PAGE

Related Product Information for FGFBP3 recombinant protein

Background: FGFBP3 is a member of the fibroblast growth factor-binding protein family. Members of this family binds and activates FGF-1 and FGF-2, thereby contributing to tumor angiogenesis. Fibroblast growth factors (FGFs) are important regulators of cell migration, proliferation and differentiation, e.g., during embryogenesis and wound healing, and under several pathological conditions including tumor growth and tumor angiogenesis. Expression of FGF-BP increases after injury to murine and human skin, in particular in keratinocytes. This upregulation is most likely achieved by major keratinocyte mitogens present at the wound site. FGFBP3 is a positive regulator of fibroblast growth factor receptor signaling pathway and vascular permeability. It interacts with 2,3,7,8-tetrachlorodibenzodioxine, benzopyrene and valproic acid. FGFBP3 also exhibits fibroblast growth factor binding (orthology) and heparin binding (orthology).

References

Abuharbeid S, et al. (2006) The fibroblast growth factor-binding protein FGF-BP. Int J Biochem Cell Biol. 38(9):1463-8.Lange LG, et al. (1976) Human liver alcohol dehydrogenase: purification, composition, and catalytic features. Biochemistry. 15(21):4687-93.Czubayko F, et al. (1997) A secreted FGF-binding protein can serve as the angiogenic switch in human cancer. Nat Med. 3(10):1137-40.

NCBI and Uniprot Product Information

• NCBI GI #: 190341093
• NCBI GeneID: 143282
• NCBI Accession #: NP_689642.3
• NCBI GenBank Nucleotide #: NM_152429.4
• UniProt Accession #: Q8TAT2; Q8NBN0; B2RD68
• Molecular Weight: 27,590 Da
• NCBI Official Full Name: fibroblast growth factor-binding protein 3
• NCBI Official Synonym Full Names: fibroblast growth factor binding protein 3
• NCBI Official Symbol: FGFBP3
• NCBI Official Synonym Symbols: FGF-BP3; C10orf13
• NCBI Protein Information: fibroblast growth factor-binding protein 3; FGFBP-3; FGF-binding protein 3
• UniProt Protein Name: Fibroblast growth factor-binding protein 3
• UniProt Gene Name: FGFBP3
• UniProt Synonym Gene Names: C10orf13; PSEC0101; FGF-BP3; FGF-binding protein 3; FGFBP-3
• UniProt Entry Name: FGFP3_HUMAN