Fish Heat Shock Protein 70 (HSP-70) ELISA Kit | HSP-70 elisa kit

Fish Heat Shock Protein 70, HSP-70 ELISA Kit

Cat. Num. AAA15817

• Gene Names: Hsp70Ab; CG18743; dhsp70; dHsp70; Dm-hsp70; DmelCG18743; DMHSP7A2; GRP78; hs70; hsp 70; Hsp 70; hsp-70; Hsp-70; hsp70; Hsp70; HSP70; hsp70 87A; hsp70(87A); Hsp70-Ab; hsp70a; hsp70A; Hsp70A; hsp70Ab; Hsp70ab; Hsp70aB; HSP70Ab; Hsp70b
• Reactivity: Fish
• Synonyms: Heat Shock Protein 70 (HSP-70); Fish Heat Shock Protein 70; HSP-70 ELISA Kit; Fish Heat Shock Protein 70 (HSP-70) ELISA Kit; HSP-70 elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish HSP-70. No significant cross-reactivity or interference between fish HSP-70 and analogues was observed.
• Sequence Length: 642
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Competitive
• Detection Range: 18.75 pg/ml-300 pg/ml
• Sensitivity: <18.75 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for HSP-70 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-70. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated HSP-70. A competitive inhibition reaction is launched between HSP-70 (Standards or samples) and biotin-conjugated HSP-70 with the pre-coated antibody specific for HSP-70. The more amount of HSP-70 in samples, the less antibody bound by biotin-conjugated HSP-70. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of HSP-70 in the sample. The color development is stopped and the intensity of the color is measured.

References

HSP70 Downregulation by Pifithrin-? Upregulates Pro-Apoptotic Changes in Stressed Fish Hepatocytes. Padmini Ekambaram, Tharani Jayachandran (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 24646137
• NCBI GeneID: 44920
• NCBI Accession #: NP_524798.2
• NCBI GenBank Nucleotide #: NM_080059.3
• UniProt Accession #: P82910; Q5BI85; Q8MT04; Q95NG7; Q9BIS5; P02825
• Molecular Weight: 70,160 Da
• NCBI Official Full Name: Heat-shock-protein-70Ab
• NCBI Official Synonym Full Names: Heat-shock-protein-70Ab
• NCBI Official Symbol: Hsp70Ab
• NCBI Official Synonym Symbols: CG18743; dhsp70; dHsp70; Dm-hsp70; DmelCG18743; DMHSP7A2; GRP78; hs70; hsp 70; Hsp 70; hsp-70; Hsp-70; hsp70; Hsp70; HSP70; hsp70 87A; hsp70(87A); Hsp70-Ab; hsp70a; hsp70A; Hsp70A; hsp70Ab; Hsp70ab; Hsp70aB; HSP70Ab; Hsp70b
• NCBI Protein Information: CG18743 gene product from transcript CG18743-RA; 87A locus; CG18743-PA; Heat-shock-protein-70Ab; Hsp70Ab-PA; heat shock 70; heat shock protein 70; heat shock protein 70Ab; heat-shock protein 70
• UniProt Protein Name: Major heat shock 70 kDa protein Aa
• UniProt Gene Name: Hsp70Aa
• UniProt Synonym Gene Names: Hsp70A; CG31366; Heat shock protein 70Aa
• UniProt Entry Name: HSP70_DROME