Standard Curve (Sample)
Zebrafish Gonadotropin Releasing Hormone (GnRH) ELISA Kit | GnRH elisa kit
Zebrafish Gonadotropin Releasing Hormone (GnRH) ELISA Kit
Reactivity
Zebrafish
Synonyms
Gonadotropin Releasing Hormone (GnRH); N/A; Zebrafish Gonadotropin Releasing Hormone (GnRH) ELISA Kit; ELISA Kit for Gonadotropin Releasing Hormone (GnRH); GnRH elisa kit
Reactivity
Zebrafish
Specificity
This assay has high sensitivity and excellent specificity for detection of GnRH. No significant cross-reactivity or interference between GnRH and analogues was observed.
Sequence Length
99
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
5.39pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GnRH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GnRH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GnRH elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GnRH in zebrafish serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GnRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GnRH and unlabeled GnRH (Standards or samples) with the pre-coated antibody specific to GnRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GnRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GnRH in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GnRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GnRH and unlabeled GnRH (Standards or samples) with the pre-coated antibody specific to GnRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GnRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GnRH in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
gonadotropin releasing hormone, partial
Similar Products
Product Notes
The Zebrafish GnRH (Catalog #AAA129537) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA129537 ELISA Kit recognizes Zebrafish GnRH. It is sometimes possible for the material contained within the vial of "Gonadotropin Releasing Hormone (GnRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.