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product-image-AAA134609_AD11.jpg Application Data

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) Recombinant Protein | VEGFR1 recombinant protein

Recombinant Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)

Gene Names
Flt1; VEGFR-1
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Vascular Endothelial Growth Factor Receptor 1 (VEGFR1); N/A; Recombinant Vascular Endothelial Growth Factor Receptor 1 (VEGFR1); VEGFR1 recombinant protein
Ordering
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-SRDKGLYT CRVKSGSSFR TFNTSVHVYE KGFISVKHRK QQVQETIAGK RSHRLSMKVK AFPSPEVVWL KDGVPATEKS ARYSVHGYSL IIKDVTAEDA GDYTILLGIK QSKLFRNLTA TLIVNVKPQI YEKSVSSLPS PPLYPLGSRQ VLTCTVYGIP QPTIKWLWHP CHYNHSKERN DFCFGSEESF ILDSSSNIGN RIEGITQRMM VIEGTNKTVS TLVV
Sequence Length
1336
Applicable Applications for VEGFR1 recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Ser303~Val524 (Accession # P53767) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Application Data

product-image-AAA134609_AD11.jpg Application Data

SDS-PAGE

product-image-AAA134609_SDS_PAGE13.jpg SDS-PAGE

Sequence

product-image-AAA134609_SEQUENCE15.jpg Sequence

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30.7kDa
NCBI Official Full Name
vascular endothelial growth factor receptor 1
NCBI Official Synonym Full Names
FMS-related tyrosine kinase 1
NCBI Official Symbol
Flt1
NCBI Official Synonym Symbols
VEGFR-1
NCBI Protein Information
vascular endothelial growth factor receptor 1; FLT-1; FMS-like tyrosine kinase 1; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor-1; Fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
UniProt Protein Name
Vascular endothelial growth factor receptor 1
UniProt Gene Name
Flt1
UniProt Synonym Gene Names
Flt-1; Vegfr1; VEGFR-1; FLT-1
UniProt Entry Name
VGFR1_RAT

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Product Notes

The VEGFR1 flt1 (Catalog #AAA134609) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the VEGFR1 flt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-SRDKGLY T CRVKSGSSFR TFNTSVHVYE KGFISVKHRK QQVQETIAGK RSHRLSMKVK AFPSPEVVWL KDGVPATEKS ARYSVHGYSL IIKDVTAEDA GDYTILLGIK QSKLFRNLTA TLIVNVKPQI YEKSVSSLPS PPLYPLGSRQ VLTCTVYGIP QPTIKWLWHP CHYNHSKERN DFCFGSEESF ILDSSSNIGN RIEGITQRMM VIEGTNKTVS TLVV. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor Receptor 1 (VEGFR1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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