VEGFR1/Flt-1 recombinant protein

Recombinant human VEGFR1/Flt-1 protein

Cat. Num. AAA48427

• Gene Names: FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: VEGFR1/Flt-1; N/A; Recombinant human VEGFR1/Flt-1 protein; VEGFR1; VEGF R1; VEGFR-1; Vascular endothelial growth factor receptor 1 isoform 1; Fms-like tyrosine kinase 1; FLT-1; FLT1; FLT; FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor; fms-related tyrosine kinase 1; vascular endothelial growth factor; vascularpermeability factor receptor; VEGFR1/Flt-1 recombinant protein

• Host: HEK293
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-328aa
• Sequence: SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HI

• Applicable Applications for VEGFR1/Flt-1 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: hIgG-Tag
• Predicted Nterm: Ser 27
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range <= 60ng/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human VEGFR1/Flt-1 inhibit proliferation of the HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for VEGFR1/Flt-1 recombinant protein

VEGFR1(Vascular endothelial growth factor receptor 1), also known as FLT-1(Fms-like tyrosine kinase 1), is belongs to the class III subfamily of receptor tyrosine kinases. While family members VEGFR1, VEGFR2and VEGFR3 are all mainly expressed on endothelial cells, only VEGFR1 is expressed on macrophages, and mainly plays inhibitory roles. Inhibitors of VEGFR are used in the treatment of cancer. It acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than does VEGFR2, but shows weaker kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Recombinant human VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for VEGFR1/Flt-1 recombinant protein

Angiogenesis; Cardiovascular

References

Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.;; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.;; Herley M.T., et al.,(1999), Biochem. Biophys. Res. Commun. 262:731-738.;; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.;;

NCBI and Uniprot Product Information

• NCBI GI #: 156104876
• NCBI GeneID: 2321
• NCBI Accession #: NP_002010.2
• NCBI GenBank Nucleotide #: NM_002019.4
• UniProt Accession #: P17948; O60722; A3E342; A3E344; A8KA71; B0LPF1; B2BF46; B2BF47; B2BF48; B3FR89; B5A923; F5H5L6
• Molecular Weight: 41,175 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1 isoform 1
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 1
• NCBI Official Symbol: FLT1
• NCBI Official Synonym Symbols: FLT; FLT-1; VEGFR1; VEGFR-1
• NCBI Protein Information: vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: FLT1
• UniProt Synonym Gene Names: FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT
• UniProt Entry Name: VGFR1_HUMAN