Sheep follicle stimulating hormone, beta polypeptide ELISA Kit | FSH elisa kit

Sheep Follicle Stimulating Hormone, FSH ELISA Kit

Cat. Num. AAA80192

• Reactivity: Sheep
• Synonyms: follicle stimulating hormone, beta polypeptide; N/A; Sheep Follicle Stimulating Hormone, FSH ELISA Kit; Sheep Follicle Stimulating Hormone (FSH) ELISA kit; follicle-stimulating hormone beta subunit; follitropin; beta chain; Sheep Follicle Stimulating Hormone (FSH); FSH elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep FSHB. No significant cross-reactivity or interference between Sheep FSHB and analogues was observed.
• Sequence Length: 129
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0 mIU/ml-70 mIU/ml
• Sensitivity: 1 mIU/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Sex hormone
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for FSH elisa kit

Introduction: Follicle-stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotropes in the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. FSH and Luteinizing hormone (LH) act synergistically in reproduction. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. Its structure is similar to those of LH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHB), which confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours. Its molecular wt is 30000.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated FSH and antibody preparation specific for FSH and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 57619324
• NCBI GeneID: 443387
• NCBI Accession #: NP_001009798.1
• NCBI GenBank Nucleotide #: NM_001009798.1
• UniProt Accession #: P01227
• Molecular Weight: 14,669 Da
• NCBI Official Full Name: follitropin subunit beta
• NCBI Official Symbol: FSHB
• NCBI Protein Information: follitropin subunit beta; FSH-B; FSH-beta; follitropin beta chain; follicle stimulating hormone beta-subunit; follicle-stimulating hormone beta subunit
• UniProt Protein Name: Follitropin subunit beta
• UniProt Gene Name: FSHB
• UniProt Synonym Gene Names: FSH-B; FSH-beta
• UniProt Entry Name: FSHB_SHEEP