Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit | GAD elisa kit

Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit

Cat. Num. AAA16638

• Gene Names: GAD1; GAD; SCP; CPSQ1
• Reactivity: Human
• Synonyms: Anti Glutamic Acid Decarboxylase Antibodies; Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit; GAD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GADAb. No significant cross-reactivity or interference between GADAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GADAb and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GAD elisa kit

Principle of the Assay: GADAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a specific antigen and an GADAb-HRP conjugate. The assay sample and buffer are incubated together with GADAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GADAb concentration since GADAb from samples and GADAb-HRP conjugate compete for the a specific antigen binding site. Since the number of sites is limited, as more sites are occupied by GADAb from the sample, fewer sites are left to bind GADAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GADAb concentration in each sample is interpolated from this standard curve.

Intended Uses: This GADAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GADAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GAD elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1247492
• NCBI GeneID: 2571
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: GAD
• NCBI Official Synonym Full Names: glutamate decarboxylase 1 (brain, 67kDa)
• NCBI Official Symbol: GAD1
• NCBI Official Synonym Symbols: GAD; SCP; CPSQ1
• NCBI Protein Information: glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 1
• UniProt Gene Name: GAD1
• UniProt Synonym Gene Names: GAD; GAD67; GAD-67
• UniProt Entry Name: DCE1_HUMAN