Standard Curve (Sample)
Human Glutamate Decarboxylase Acid ELISA Kit | GAD elisa kit
Human Glutamate Decarboxylase Acid ELISA Kit
Gene Names
GAD1; GAD; SCP; CPSQ1
Reactivity
Human
Synonyms
Glutamate Decarboxylase Acid; N/A; Human Glutamate Decarboxylase Acid ELISA Kit; GAD elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GAD elisa kit
Intended Uses: This GAD ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GAD in the sample, this GAD ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GAD concentration. The concentration of GAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This GAD enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GAD. Standards or samples are then added to the microtiter plate wells and GAD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GAD present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GAD are added to each well to "sandwich" the GAD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GAD and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Principle of the Assay: This GAD enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for GAD. Standards or samples are then added to the microtiter plate wells and GAD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GAD present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GAD are added to each well to "sandwich" the GAD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain GAD and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Product Categories/Family for GAD elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,440 Da
NCBI Official Full Name
GAD
NCBI Official Synonym Full Names
glutamate decarboxylase 1 (brain, 67kDa)
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
NCBI Protein Information
glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
UniProt Entry Name
DCE1_HUMAN
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Product Notes
The Human GAD gad1 (Catalog #AAA83222) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA83222 ELISA Kit recognizes Human GAD. It is sometimes possible for the material contained within the vial of "Glutamate Decarboxylase Acid, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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