Human growth arrest and DNA-damage-inducible, alpha ELISA Kit | GADD45A elisa kit

Human Growth arrest and DNA damage-inducible protein GADD45 alpha, GADD45A ELISA Kit

Cat. Num. AAA112240

• Gene Names: GADD45A; DDIT1; GADD45
• Reactivity: Human
• Synonyms: growth arrest and DNA-damage-inducible, alpha; N/A; Human Growth arrest and DNA damage-inducible protein GADD45 alpha, GADD45A ELISA Kit; Human Growth arrest and DNA damage-inducible protein GADD45 alpha (GADD45A) ELISA kit; RP5-975D15.1; DDIT1; GADD45; DNA damage-inducible transcript 1; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible; alpha; GADD45A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GADD45A. No significant cross-reactivity or interference between human GADD45A and analogues was observed.
• Sequence Length: 131
• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml
• Sensitivity: < 6.25 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for GADD45A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GADD45A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GADD45A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GADD45A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GADD45A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 315075323
• NCBI GeneID: 1647
• NCBI Accession #: NP_001186670.1
• NCBI GenBank Nucleotide #: NM_001199741.1
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 18,336 Da
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
• NCBI Official Synonym Full Names: growth arrest and DNA-damage-inducible, alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1
• UniProt Entry Name: GA45A_HUMAN