N-acetylgalactosamine-6-sulfatase Recombinant Protein | GALNS recombinant protein

Recombinant Human N-acetylgalactosamine-6-sulfatase

Cat. Num. AAA18470

• Gene Names: GALNS; GAS; MPS4A; GalN6S; GALNAC6S
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: N-acetylgalactosamine-6-sulfatase; Recombinant Human N-acetylgalactosamine-6-sulfatase; Chondroitinsulfatase; Chondroitinase; Galactose-6-sulfate sulfatase; GalN6S; N-acetylgalactosamine-6-sulfate sulfatase; GalNAc6S sulfatase; GALNS recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 27-522aa; Full Length of Mature Protein
• Sequence: APQPPNILLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGLLFPNFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIVGGIPDSEQLLPELLKKAGYVSKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKARPNIPVYRDWEMVGRYYEEFPINLKTGEANLTQIYLQEALDFIKRQARHHPFFLYWAVDATHAPVYASKPFLGTSQRGRYGDAVREIDDSIGKILELLQDLHVADNTFVFFTSDNGAALISAPEQGGSNGPFLCGKQTTFEGGMREPALAWWPGHVTAGQVSHQLGSIMDLFTTSLALAGLTPPSDRAIDGLNLLPTLLQGRLMDRPIFYYRGDTLMAATLGQHKAHFWTWTNSWENFRQGIDFCPGQNVSGVTTHNLEDHTKLPLIFHLGRDPGERFPLSFASAEYQEALSRITSVVQQHQEALVPAQPQLNVCNWAVMNWAPPGCEKLGKCLTPPESIPKKCLWSH

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Product Categories/Family for GALNS recombinant protein

Signal Transduction

References

Morquio disease isolation, characterization and expression of full-length cDNA for human N-acetylgalactosamine-6-sulfate sulfatase.Tomatsu S., Fukuda S., Masue M., Sukegawa K., Fukao T., Yamagishi A., Hori T., Iwata H., Ogawa T., Nakashima Y., Hanyu Y., Hashimoto T., Titani K., Oyama R., Suzuki M., Yagi K., Hayashi Y., Orii T.Biochem. Biophys. Res. Commun. 181:677-683(1991) Morquio A syndrome cloning, sequence, and structure of the human N-acetylgalactosamine 6-sulfatase (GALNS) gene.Morris C.P., Guo X.H., Apostolou S., Hopwood J.J., Scott H.S.Genomics 22:652-654(1994) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) The structure of human GALNS reveals the molecular basis for mucopolysaccharidosis IV A.Rivera-Colon Y., Schutsky E.K., Kita A.Z., Garman S.C.J. Mol. Biol. 423:736-751(2012) Mucopolysaccharidosis type IVA. N-acetylgalactosamine-6-sulfate sulfatase exonic point mutations in classical Morquio and mild cases.Fukuda S., Tomatsu S., Masue M., Sukegawa K., Iwata H., Ogawa T., Nakashima Y., Hori T., Yamagishi A., Hanyu Y., Morooka K., Kiman T., Hashimoto T., Orii T.J. Clin. Invest. 90:1049-1053(1992) Mucopolysaccharidosis IVA identification of a common missense mutation I113F in the N-Acetylgalactosamine-6-sulfate sulfatase gene.Tomatsu S., Fukuda S., Cooper A., Wraith J.E., Maruf Rezvi G., Yamagishi A., Yamada N., Kato Z., Isogai K., Sukegawa K., Kondo N., Suzuki Y., Shimozawa N., Orii T.Am. J. Hum. Genet. 57:556-563(1995) Mucopolysaccharidosis IVA screening and identification of mutations of the N-acetylgalactosamine-6-sulfate sulfatase gene.Ogawa T., Tomatsu S., Fukuda S., Yamagishi A., Maruf Rezvi G., Sukegawa K., Kondo N., Suzuki Y., Shimozawa N., Orii T.Hum. Mol. Genet. 4:341-349(1995) Mucopolysaccharidosis type IVA identification of six novel mutations among non-Japanese patients.Tomatsu S., Fukuda S., Cooper A., Wraith J.E., Maruf Rezvi G., Yamagishi A., Yamada N., Kato Z., Isogai K., Sukegawa K., Kondo N., Suzuki Y., Shimozawa N., Orii T.Hum. Mol. Genet. 4:741-743(1995) Two new mutations, Q473X and N487S, in a Caucasian patient with mucopolysaccharidosis IVA (Morquio disease) .Tomatsu S., Fukuda S., Cooper A., Wraith J.E., Yamada N., Isogai K., Kato Z., Sukegawa K., Kondo N., Suzuki Y., Shimozawa N., Orii T.Hum. Mutat. 6:195-196(1995) Mucopolysaccharidosis IVA four new exonic mutations in patients with N-acetylgalactosamine-6-sulfate sulfatase deficiency.Tomatsu S., Fukuda S., Yamagishi A., Cooper A., Wraith J.E., Hori T., Kato Z., Yamada N., Isogai K., Sukegawa K., Kondo N., Suzuki Y., Shimozawa N., Orii T.Am. J. Hum. Genet. 58:950-962(1996) Heteroallelic missense mutations of the galactosamine-6-sulfate sulfatase (GALNS) gene in a mild form of Morquio disease (MPS IVA) .Cole D.E.C., Fukuda S., Gordon B.A., Rip J.W., Lecouteur A.N., Rupar C.A., Tomatsu S., Ogawa T., Sukegawa K., Orii T.3.3.CO;2-V>Am. J. Med. Genet. 63:558-565(1996) Identification of 31 novel mutations in the N-acetylgalactosamine-6-sulfatase gene reveals excessive allelic heterogeneity among patients with Morquio A syndrome.Bunge S., Kleijer W.J., Tylki-Szymanska A., Steglich C., Beck M., Tomatsu S., Fukuda S., Poorthuis B.J.H.M., Czartoryska B., Orii T., Gal A.3.3.CO;2-M>Hum. Mutat. 10:223-232(1997) Fourteen novel mucopolysaccharidosis IVA producing mutations in GALNS gene.Tomatsu S., Fukuda S., Cooper A., Wraith J.E., Ferreira P., di Natale P., Tortora P., Fujimoto A., Kato Z., Yamada N., Isogai K., Yamagishi A., Sukegawa K., Suzuki Y., Shimozawa N., Kondo N., Sly W.S., Orii T.3.0.CO;2-B>Hum. Mutat. 10:368-375(1997) Molecular heterogeneity in mucopolysaccharidosis IVA in Australia and Northern Ireland nine novel mutations including T312S, a common allele that confers a mild phenotype.Yamada N., Fukuda S., Tomatsu S., Muller V., Hopwood J.J., Nelson J., Kato Z., Yamagishi A., Sukegawa K., Kondo N., Orii T.3.0.CO;2-J>Hum. Mutat. 11:202-208(1998) Fifteen polymorphisms in the N-acetylgalactosamine-6-sulfate sulfatase (GALNS) gene diagnostic implications in Morquio disease.Tomatsu S., Fukuda S., Cooper A., Wraith J.E., Yamagishi A., Kato Z., Yamada N., Isogai K., Sukegawa K., Suzuki Y., Shimozawa N., Kondo N., Orii T.Hum. Mutat. Suppl. 1:S42-S46(1998) Mutation and polymorphism spectrum of the GALNS gene in mucopolysaccharidosis IVA (Morquio A) .Tomatsu S., Montano A.M., Nishioka T., Gutierrez M.A., Pena O.M., Tranda Firescu G.G., Lopez P., Yamaguchi S., Noguchi A., Orii T.Hum. Mutat. 26:500-512(2005) The consensus coding sequences of human breast and colorectal cancers.Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.Science 314:268-274(2006) Molecular testing of 163 patients with Morquio A (Mucopolysaccharidosis IVA) identifies 39 novel GALNS mutations.Morrone A., Tylee K.L., Al-Sayed M., Brusius-Facchin A.C., Caciotti A., Church H.J., Coll M.J., Davidson K., Fietz M.J., Gort L., Hegde M., Kubaski F., Lacerda L., Laranjeira F., Leistner-Segal S., Mooney S., Pajares S., Pollard L., Ribeiro I., Wang R.Y., Miller N.Mol. Genet. Metab. 112:160-170(2014)

NCBI and Uniprot Product Information

• NCBI GI #: 4503899
• NCBI GeneID: 2588
• NCBI Accession #: NP_000503.1
• NCBI GenBank Nucleotide #: NM_000512.4
• UniProt Accession #: P34059; Q86VK3
• Molecular Weight: 57.4 kDa
• NCBI Official Full Name: N-acetylgalactosamine-6-sulfatase
• NCBI Official Synonym Full Names: galactosamine (N-acetyl)-6-sulfatase
• NCBI Official Symbol: GALNS
• NCBI Official Synonym Symbols: GAS; MPS4A; GalN6S; GALNAC6S
• NCBI Protein Information: N-acetylgalactosamine-6-sulfatase
• UniProt Protein Name: N-acetylgalactosamine-6-sulfatase
• UniProt Gene Name: GALNS
• UniProt Synonym Gene Names: Chondroitinase; GalN6S; GalNAc6S sulfatase
• UniProt Entry Name: GALNS_HUMAN