Standard Curve (Sample)
Human glycyl-tRNA synthetase ELISA Kit | GARS elisa kit
Human Glycyl-tRNA synthetase, GARS ELISA Kit
Gene Names
GARS; HMN5; CMT2D; DSMAV; GlyRS; SMAD1
Reactivity
Human
Synonyms
glycyl-tRNA synthetase; N/A; Human Glycyl-tRNA synthetase, GARS ELISA Kit; Human Glycyl-tRNA synthetase (GARS) ELISA kit; CMT2D; DSMAV; GlyRS; HMN5; SMAD1; Charcot-Marie-Tooth neuropathy 2D; Charcot-Marie-Tooth neuropathy; neuronal type; D; glycine tRNA ligase; GARS elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GARS. No significant cross-reactivity or interference between Human GARS and analogues was observed.
Sequence Length
739
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Amino-acid biosynthesis and metabolism
Protein Biological Process 3
Protein biosynthesis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GARS elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GARS bound in the initial step. The color development is stopped and the intensity of the color is measured
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,166 Da
NCBI Official Full Name
glycine--tRNA ligase
NCBI Official Synonym Full Names
glycyl-tRNA synthetase
NCBI Official Symbol
GARS
NCBI Official Synonym Symbols
HMN5; CMT2D; DSMAV; GlyRS; SMAD1
NCBI Protein Information
glycine--tRNA ligase; AP-4-A synthetase; glycine tRNA ligase; Charcot-Marie-Tooth neuropathy 2D; diadenosine tetraphosphate synthetase; Charcot-Marie-Tooth neuropathy, neuronal type, D
UniProt Protein Name
Glycine--tRNA ligase
UniProt Gene Name
GARS
UniProt Synonym Gene Names
AP-4-A synthetase; GlyRS
UniProt Entry Name
SYG_HUMAN
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Product Notes
The Human GARS gars (Catalog #AAA112390) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112390 ELISA Kit recognizes Human GARS. It is sometimes possible for the material contained within the vial of "glycyl-tRNA synthetase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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