GAS-6 Recombinant Protein | GAS6/AXLLG recombinant protein

Recombinant Human GAS-6 Protein

Cat. Num. AAA283406

• Purity: >90% by SDS-PAGE.
• Synonyms: GAS-6; N/A; Recombinant Human GAS-6 Protein; GAS6, AXLLG, Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand; GAS6/AXLLG recombinant protein

• Host: HEK293 cells
• Purity/Purification: >90% by SDS-PAGE.
• Form/Format: Lyophilized from a 0.22 um filtered solution of PBS, pH7.4.
• Sequence: MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

• Species: human
• Tag: C-His
• Endotoxin: <1 EU/ug of the protein by LAL method.
• Bio-Activity: Immobilized Human GAS6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Axl with the EC₅₀ of 7.5ng/ml determined by ELISA.
• Preparation and Storage: Store at -20 degree C. Store the lyophilized protein at -20 degree C to -80 degree C up to 1 year from the date of receipt.; After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.

Application Data

(Recombinant Human GAS-6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.)

SDS-PAGE

(Recombinant Human GAS-6 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 70-75 kDa.)

Related Product Information for GAS6/AXLLG recombinant protein

GAS-6 is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG). Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis, cell proliferation, platelet-mediated thrombosis, retinal epithelial cell phagocytosis of outer rod segments, inhibition of VEGF-induced endothelial cell chemotaxis, and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells. Several of these effects have been shown to require gamma -carboxylation of the Gla domain.

Product Categories/Family for GAS6/AXLLG recombinant protein

Cytokines & Cytokine receptors; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4557617
• NCBI GeneID: 2621
• NCBI Accession #: NP_000811.1
• NCBI GenBank Nucleotide #: NM_000820.2
• UniProt Accession #: Q14393; Q6IMN1; Q7Z7N3; B3KRQ7; B3KVL4; E9PBL7
• UniProt Protein Name: Growth arrest-specific protein 6
• UniProt Gene Name: GAS6
• UniProt Synonym Gene Names: AXLLG; GAS-6
• UniProt Entry Name: GAS6_HUMAN