Canine Guanosine 5-triphosphate Cyclohydrolase1 ELISA Kit | GCH1 elisa kit

Canine Guanosine 5-triphosphate Cyclohydrolase1 ELISA Kit

Cat. Num. AAA93437

• Gene Names: GCH1; GCH; DYT5; DYT14; DYT5a; GTPCH1; HPABH4B; GTP-CH-1
• Reactivity: Canine
• Synonyms: Guanosine 5-triphosphate Cyclohydrolase1; N/A; Canine Guanosine 5-triphosphate Cyclohydrolase1 ELISA Kit; GCH1 elisa kit

• Reactivity: Canine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL

• Intended Uses: This GCH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GCH1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GCH1 elisa kit

Principle of the Assay: GCH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCH1 antibody and an GCH1-HRP conjugate. The assay sample and buffer are incubated together with GCH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCH1 concentration since GCH1 from samples and GCH1-HRP conjugate compete for the anti-GCH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCH1 from the sample, fewer sites are left to bind GCH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCH1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GCH1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 66932972
• NCBI GeneID: 2643
• NCBI Accession #: NP_001019242.1
• NCBI GenBank Nucleotide #: NM_001024071.1
• Molecular Weight: 25,775 Da
• NCBI Official Full Name: GTP cyclohydrolase 1 isoform 3
• NCBI Official Synonym Full Names: GTP cyclohydrolase 1
• NCBI Official Symbol: GCH1
• NCBI Official Synonym Symbols: GCH; DYT5; DYT14; DYT5a; GTPCH1; HPABH4B; GTP-CH-1
• NCBI Protein Information: GTP cyclohydrolase 1; GTP-CH-I; dystonia 14; GTP cyclohydrolase I; guanosine 5'-triphosphate cyclohydrolase I
• UniProt Protein Name: GTP cyclohydrolase 1
• UniProt Gene Name: GCH1
• UniProt Synonym Gene Names: DYT5; GCH; GTP-CH-I
• UniProt Entry Name: GCH1_HUMAN