Standard Curve (Sample)
Porcine Glucokinase ELISA Kit | GCK elisa kit
Porcine Glucokinase ELISA Kit
Gene Names
Gck; GLUKA; RNGK2
Reactivity
Porcine
Synonyms
Glucokinase; N/A; Porcine Glucokinase ELISA Kit; GCK elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GCK elisa kit
Intended Uses: This GCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GCK. This ELISA kit for research use only!
Principle of the Assay: GCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCK antibody and an GCK-HRP conjugate. The assay sample and buffer are incubated together with GCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCK concentration since GCK from samples and GCK-HRP conjugate compete for the anti-GCK antibody binding site. Since the number of sites is limited, as more sites are occupied by GCK from the sample, fewer sites are left to bind GCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCK antibody and an GCK-HRP conjugate. The assay sample and buffer are incubated together with GCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCK concentration since GCK from samples and GCK-HRP conjugate compete for the anti-GCK antibody binding site. Since the number of sites is limited, as more sites are occupied by GCK from the sample, fewer sites are left to bind GCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,919 Da
NCBI Official Full Name
glucokinase isoform 2
NCBI Official Synonym Full Names
glucokinase
NCBI Official Symbol
Gck
NCBI Official Synonym Symbols
GLUKA; RNGK2
NCBI Protein Information
glucokinase; HK4; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; glucokinase variant 3
UniProt Protein Name
Glucokinase
UniProt Gene Name
Gck
UniProt Synonym Gene Names
HK IV; HK4
UniProt Entry Name
HXK4_RAT
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Product Notes
The Porcine GCK gck (Catalog #AAA92840) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA92840 ELISA Kit recognizes Porcine GCK. It is sometimes possible for the material contained within the vial of "Glucokinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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