Porcine Granulocyte Colony Stimulating Factor ELISA Kit | GCSF elisa kit

Porcine Granulocyte Colony Stimulating Factor ELISA Kit

Cat. Num. AAA16776

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Reactivity: Porcine
• Synonyms: Granulocyte Colony Stimulating Factor; Porcine Granulocyte Colony Stimulating Factor ELISA Kit; GCSF elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of GCSF. No significant cross-reactivity or interference between GCSF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCSF and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for GCSF elisa kit

Principle of the Assay: GCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GCSF antibody and an GCSF-HRP conjugate. The assay sample and buffer are incubated together with GCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSF concentration since GCSF from samples and GCSF-HRP conjugate compete for the anti-GCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSF from the sample, fewer sites are left to bind GCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSF concentration in each sample is interpolated from this standard curve.

Intended Uses: This GCSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GCSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GCSF elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 297660573
• NCBI GeneID: 1440
• UniProt Accession #: P09919; Q5FBY7; Q6FH65; Q8N4W3
• Molecular Weight: 22,293 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN