Mouse Granulocyte Colony Stimulating Factor Receptor ELISA Kit | GCSFR elisa kit
Mouse Granulocyte Colony Stimulating Factor Receptor ELISA Kit
Gene Names
CSF3R; CD114; GCSFR
Reactivity
Mouse
Synonyms
Granulocyte Colony Stimulating Factor Receptor; N/A; Mouse Granulocyte Colony Stimulating Factor Receptor ELISA Kit; GCSFR elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This G-CSFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse G-CSFR. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for GCSFR elisa kit
Principle of the Assay: G-CSFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G-CSFR antibody and an G-CSFR-HRP conjugate. The assay sample and buffer are incubated together with G-CSFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G-CSFR concentration since G-CSFR from samples and G-CSFR-HRP conjugate compete for the anti-G-CSFR antibody binding site. Since the number of sites is limited, as more sites are occupied by G-CSFR from the sample, fewer sites are left to bind G-CSFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G-CSFR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GCSFR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
86,609 Da
NCBI Official Full Name
granulocyte colony-stimulating factor receptor
NCBI Official Synonym Full Names
colony stimulating factor 3 receptor (granulocyte)
NCBI Official Symbol
CSF3R
NCBI Official Synonym Symbols
CD114; GCSFR
NCBI Protein Information
granulocyte colony-stimulating factor receptor; G-CSF-R; CD114 antigen; G-CSF receptor
UniProt Protein Name
Granulocyte colony-stimulating factor receptor
UniProt Gene Name
CSF3R
UniProt Synonym Gene Names
GCSFR; G-CSF receptor; G-CSF-R
UniProt Entry Name
CSF3R_HUMAN