Human Anti Ganglioside D1a Antibody ELISA Kit | GD1a elisa kit

Human Anti Ganglioside D1a Antibody ELISA Kit

Cat. Num. AAA17098

• Reactivity: Human
• Synonyms: Anti Ganglioside D1a Antibody; N/A; Human Anti Ganglioside D1a Antibody ELISA Kit; GD1a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-GD1a. No significant cross-reactivity or interference between Anti-GD1a and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-GD1a and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GD1a elisa kit

Principle of the Assay: Anti-GD1a ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside D1a antigen and an Anti-GD1a-HRP conjugate. The assay sample and buffer are incubated together with Anti-GD1a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GD1a concentration since Anti-GD1a from samples and Anti-GD1a-HRP conjugate compete for the Ganglioside D1a antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-GD1a from the sample, fewer sites are left to bind Anti-GD1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GD1a concentration in each sample is interpolated from this standard curve.

Intended Uses: This Anti-GD1a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-GD1a. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GD1a elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 40388265
• Molecular Weight: 46,635 Da
• NCBI Official Full Name: GD1A, partial
• UniProt Protein Name: Histidinol-phosphate aminotransferase 2, chloroplastic
• UniProt Gene Name: HISN6B
• UniProt Synonym Gene Names: GD1-A; HPA2; At1g71920; F17M19.7
• UniProt Entry Name: HIS6B_ARATH