General Isocitrate Lyase Assay Kit | ICL assay kit
Isocitrate Lyase Microplate Assay Kit
Gene Names
ICL; isocitrate lyase
Reactivity
General
Applications
Functional Assay
Synonyms
Isocitrate Lyase; N/A; Isocitrate Lyase Microplate Assay Kit; EC 4.1.3.1; Isocitrate lyase; ICL; Isocitrase; Isocitratase; Isocitrate glyoxylate-lyase; Isocitritase; ICL assay kit
Reactivity
General
Sequence Length
576
Samples
Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
Detection Range
4 umol/L - 400 umol/L
Applicable Applications for ICL assay kit
Functional Assay
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Related Product Information for ICL assay kit
Background: Isocitrate lyase (EC 4.1.3.1), or ICL, is an enzyme in the glyoxylate cycle that catalyzes the cleavage of isocitrate to succinate and glyoxylate. Together with malate synthase, it bypasses the two decarboxylation steps of the tricarboxylic acid cycle (TCA cycle) and is used by bacteria, fungi, and plants. The assay is initiated with the enzymatic decomposition of the Isocitric acid by Isocitrate lyase. The enzyme catalysed reaction product NADH can be measured at a colorimetric readout at 340 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,245 Da
NCBI Official Full Name
isocitrate lyase
NCBI Official Symbol
ICL
NCBI Official Synonym Symbols
isocitrate lyase
NCBI Protein Information
isocitrate lyase
UniProt Protein Name
Isocitrate lyase
UniProt Gene Name
ICL
UniProt Synonym Gene Names
ICL
UniProt Entry Name
ACEA_ARATH