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product-image-AAA264776_SC15.jpg Standard Curve (Sample)

General Lactate Assay Kit

Lactate Microplate Assay Kit

Reactivity
General
Applications
Functional Assay
Synonyms
Lactate; N/A; Lactate Microplate Assay Kit; Lactate assay kit
Ordering
Reactivity
General
Samples
Serum, Plasma, Cell culture media, Other biological fluids Samples
Detection Range
0.1 mmol/L - 100 mmol/L
Applicable Applications for Lactate assay kit
FA (Functional Assay)
Preparation and Storage
Shipped and store at 4 degree C for 6 months.

Standard Curve (Sample)

product-image-AAA264776_SC15.jpg Standard Curve (Sample)
Related Product Information for Lactate assay kit
Background: L-lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in a process of fermentation during normal metabolism and exercise. It does not increase in concentration until the rate of lactate production exceeds the rate of lactate removal, which is governed by a number of factors, including monocarboxylate transporters, concentration and isoform of LDH, and oxidative capacity of tissues. The concentration of blood lactate is usually 1- 2 mM at rest, but can rise to over 20 mM during intense exertion and as high as 25 mM afterward. In addition to other biological roles, L-lactic acid is the primary endogenous agonist of hydroxycarboxylic acid receptor 1 (HCA1), which is a Gi/o-coupled G protein-coupled receptor (GPCR). Lactate Microplate Assay Kit is a sensitive assay for determining lactate content in various samples. The kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 450 nm, is proportionate to the lactate concentration in the sample.

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Product Notes

The General Lactate (Catalog #AAA264776) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA264776 Assay Kit recognizes General Lactate. AAA Biotech's Lactate can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the Lactate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lactate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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