Standard Curve (Sample)
General Pyruvate Carboxylase Assay Kit
Pyruvate Carboxylase Microplate Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Pyruvate Carboxylase; N/A; Pyruvate Carboxylase Microplate Assay Kit; pyruvic carboxylase; Pyruvate Carboxylase assay kit
Reactivity
General
Samples
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
Detection Range
4 umol/L - 400 umol/L
Applicable Applications for Pyruvate Carboxylase assay kit
FA (Functional Assay)
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Pyruvate Carboxylase assay kit
Background: Pyruvate carboxylase (EC 6.4.1.1) catalyzes the carboxylation of pyruvate to oxaloacetate, which is the precursor for the biosynthesis of many C4 intermediates and is used in gluconeogenesis, biosynthesis of amino acids, and fat metabolism. Pyruvate carboxylase belongs to the family of biotin-dependent carboxylases and is composed of four identical subunits (?130 kDa each) organized as a tetramer. It is present in many organisms including bacteria, fungi, plants, and animals. Pyruvate carboxylase is situated in mitochondria in most eukaryotic organisms. Biotin is an important regulator of pyruvate carboxylase activity. Long-term regulation of this enzyme in yeast can be realized by controlling the availability of biotin. Additionally, acetyl-CoA and l-aspartate could be used as the activator and inhibitor, respectively, for short-term regulation. Pyruvate Carboxylase Microplate Assay Kit provides a simple and direct procedure for measuring pyruvate carboxylase levels in a variety of samples. The formation of oxaloacetate is monitored spectrophotometrically in a malate dehydrogenase coupled system. The reaction velocity is measured as a decrease in A340 resulting from the oxidation of NADH.
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Product Notes
The General Pyruvate Carboxylase (Catalog #AAA264810) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA264810 Assay Kit recognizes General Pyruvate Carboxylase. AAA Biotech's Pyruvate Carboxylase can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the Pyruvate Carboxylase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Carboxylase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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